Chemical-library composition and method

• US 20030008323A1
• Filed: 04/09/2002
• Published: 01/09/2003
• Est. Priority Date: 04/15/1999
• Status: Active Grant

• Current Assignee: Millipore Corporation
• Original Assignee: Vitra Biosciences, LLC
• Inventors: Zarowitz, Michael A., Hyun, William C., Goldbard, Simon, Ravkin, Ilya
• Granted Patent: US 6,908,737 B2
• Time in Patent Office: Days
• US Class Current: 435/7.1
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/0052 in the shape of elongated tubes
• CPC Class Codes: B01J 2219/00524 in the shape of fiber bundles
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/0054 Means for coding or tagging the apparatus or the reagents
• CPC Class Codes: B01J 2219/00545 Colours
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00592 Split-and-pool, mix-and-divide processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00657 One-dimensional arrays
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00673 Slice arrays
• CPC Class Codes: B01J 2219/00702 : Processes involving means for analysing and characterising the products
• CPC Class Codes: B01J 2219/00707 : separated from the reactor apparatus
• CPC Class Codes: B01J 2219/0072 : Organic compounds
• CPC Class Codes: B01J 2219/00722 : Nucleotides
• CPC Class Codes: B01J 2219/00725 : Peptides
• CPC Class Codes: C07B 2200/11 : Compounds covalently bound to a solid support
• CPC Class Codes: C07K 1/047 : Simultaneous synthesis of different peptide species; Peptide libraries
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: G01N 33/54366 : Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/587 : Nanoparticles
• CPC Class Codes: Y10S 435/808 : Optical sensing apparatus
• CPC Class Codes: Y10S 435/968 : High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
• CPC Class Codes: Y10S 436/807 : Apparatus included in process claim, e.g. physical support structures

Abstract

A method for multiplexed detection and quantification of analytes by reacting them with probe molecules attached to specific and identifiable carriers. These carriers can be of different size, shape, color, and composition. Different probe molecules are attached to different types of carriers prior to analysis. After the reaction takes place, the carriers can be automatically analyzed. This invention obviates cumbersome instruments used for the deposition of probe molecules in geometrically defined arrays. In the present invention, the analytes are identified by their association with the defined carrier, and not (or not only) by their position. Moreover, the use of carriers provides a more homogenous and reproducible representation for probe molecules and reaction products than two-dimensional imprinted arrays or DNA chips.

Claims

1. A system for conducting a multiplexed experiment comprising

a set of microcarriers including multiple classes of microcarriers,

a first class of microcarriers each having a first code configuration characterized by a first optically distinguishable combination of colored spatial indicia, and

a second class of microcarriers each having a second code configuration characterized by a second optically distinguishable combination of colored spatial indicia.

2. The system of claim 1, wherein the colored spatial indicia are three-dimensional.

3. The system of claim 1, wherein each microcarrier is formed at least partially from multiple fibers.

4. The system of claim 3, wherein the fibers are fused.

5. The system of claim 3, wherein the fibers comprise glass.

6. The system of claim 3, wherein the fibers comprise plastic.

7. The system of claim 3, wherein the fibers in each class of microcarrier are distinctively colored.

8. The system of claim 1, wherein each microcarrier has a generally flat shape with two substantially parallel planar sides so that the microcarrier tends to lay on one of the planar sides, the code being detectable on the other planar side.

9. The system of claim 1, further comprising

compounds attached to the microcarriers, wherein the code on a microcarrier identifies the compound carried by the microcarrier.

10. The system of claim 1, further comprising

cells attached to at least some of the microcarriers, wherein the code on the microcarrier identifies a characteristic of a cell carried by the microcarrier.

11. The system of claim 1, wherein the set of microcarriers includes at least three classes of microcarriers, each class of microcarrier having a distinctive code configuration.

12. The system of claim 1, wherein the microcarriers are formed from a flat ribbon of parallel fused colored fibers.

13. A system for conducting a multi-plexed experiment comprising

a set of microcarriers including multiple classes of micro carriers,

a first class of microcarriers each having a first code configuration characterized by a first optically distinguishable combination of colored fibers, and

a second class of microcarriers each having a second code configuration characterized by a second optically distinguishable combination of colored fibers.

14. The system of claim 13, wherein the fibers in each microcarrier are fused together.

15. The system of claim 13, wherein the fibers are glass.

16. The system of claim 13, wherein the fibers are plastic.

17. The system of claim 13, wherein the set of microcarriers includes at least three classes of microcarriers, each class of microcarrier having a distinctive code configuration.

18. The system of claim 13, wherein each microcarrier has a generally flat shape with two substantially parallel planar sides, so that the microcarrier tends to lay on one of the planar sides, the code being detectable on the other planar side.

19. The system of claim 13 further comprising

compounds attached to the microcarrier, wherein the code on a microcarrier identifies the compound carried by the microcarrier.

20. The system of claim 13 further comprising

cells attached to at least some of the microcarriers, wherein the code on a microcarrier identifies a characteristic of a cell carried by the microcarrier.

21. The system of claim 13, wherein the microcarriers are formed from a flat ribbon of parallel fused colored fibers.

22. A system for conducting a multiplexed experiment comprising

a set of microcarriers including multiple classes of microcarriers,

a first class of microcarriers each having a first optically detectable code, and

a second class of microcarriers each having a second optically detectable code, wherein each microcarrier has a generally flat shape with two substantially parallel planar sides, so that the microcarrier tends to lay on one of the planar sides, the code being detectable on the other planar side.

23. The system of claim 22, wherein each microcarrier is at least partially formed by a combination of differently-colored fibers, the combination of differently color fibers defining the code.

24. The system of claim 23, wherein the fibers in each microcarrier are fused together.

25. The system of claim 23, wherein the fibers in each microcarrier comprise glass.

26. The system of claim 23, wherein the fibers in each microcarrier comprise plastic.

27. The system of claim 22 further comprising

compounds attached to microcarriers, wherein the code on each microcarrier identifies the compound carried by the microcarrier.

28. The system of claim 22, wherein each microcarrier is formed from a flat ribbon of parallel fused colored fibers.