Disposable cell culture vessel
First Claim
Patent Images
1. A vessel for cell culture comprising:
- a headplate having a circumferential edge; and
a collapsible bag with an inner surface, an outer surface and a top periphery, with said top periphery of said bag sealed to said edge of said headplate.
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Accused Products
Abstract
The present invention relates to an improved vessel for cell culture and methods for its use. The vessel of the present invention comprises a collapsible bag with an inner surface, an outer surface and a top periphery and a headplate having a circumferential edge wherein the top periphery of the bag is sealed to the edge of the headplate. The present invention also relates to an impeller for use with the vessel and a method for culturing cells.
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Citations
25 Claims
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1. A vessel for cell culture comprising:
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a headplate having a circumferential edge; and
a collapsible bag with an inner surface, an outer surface and a top periphery, with said top periphery of said bag sealed to said edge of said headplate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 16, 17, 18, 19)
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13. A vessel for cell culture comprising:
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a headplate;
a pre-sterilized collapsible bag sealed to said headplate;
an impeller comprising a hollow flexible shaft connected to said headplate;
two flexible blades attached to said impeller; and
a constriction device o-ring disposed on said flexible shaft.
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15. An impeller comprising a hollow flexible shaft having a top region and a bottom region, said bottom region having a flexible blade.
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20. A method of mixing a fluid comprising the steps of:
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providing a vessel comprising a collapsible bag containing an impeller comprised of a hollow flexible shaft;
inserting a magnet into said hollow shaft of said impeller;
introducing an external magnetic source to interact with said magnet and cause said magnet and said hollow shaft to move; and
removing said magnet from said hollow shaft of said impeller. - View Dependent Claims (21, 22, 25)
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23. The method of culturing cells in a pre-sterilized vessel comprising a collapsible bag with a headplate and an impeller comprised of a hollow flexible shaft having a top region and a bottom region, wherein said top region is connected to said headplate and wherein said bottom region comprises a flexible blade comprising the steps of the method of:
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inserting a magnet into said hollow shaft of said impeller;
introducing a cell line and media into said vessel;
allowing said cell line to proliferate;
removing said cell line and media from said vessel;
removing said magnet from said hollow shaft of said impeller; and
disposing of said vessel.
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24. A method of culturing cells in a collapsible vessel containing an impeller having a hollow shaft, the method comprising the steps of:
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inserting a magnet into said hollow shaft;
introducing an external magnetic source to interact with said magnet and cause said magnet and said hollow shaft to move; and
removing said magnet from said hollow shaft.
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Specification