Methods of making and using substrate surfaces having covalently bound polyelectrolyte films
First Claim
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1. A method for stably associating a polyelectrolyte layer with a substrate surface, said method comprising:
- (a) producing a polyelectrolyte layer on at least one surface of a substrate;
(b) contacting said polyelectrolyte layer with a bifunctional molecule comprising;
(i) a first moiety that stably associates with said polyelectrolyte layer, and (ii) a second moiety that stably associates with said substrate surface;
wherein said contacting occurs under conditions sufficient to stably associate said polyectrolyte layer to said substrate surface.
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Abstract
Methods for stably associating a polyelectrolyte coating or film to a substrate surface, as well as the coated substrates produced thereby, are disclosed herein. In the subject methods, substrate surfaces are coated with a polyelectrolyte, and the coated substrate surfaces are treated with a bifunctional molecule under conditions sufficient to stably associate the film to the array surface. The subject methods find use in a variety of different applications, including the production of mircoarrarys.
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Citations
40 Claims
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1. A method for stably associating a polyelectrolyte layer with a substrate surface, said method comprising:
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(a) producing a polyelectrolyte layer on at least one surface of a substrate;
(b) contacting said polyelectrolyte layer with a bifunctional molecule comprising;
(i) a first moiety that stably associates with said polyelectrolyte layer, and (ii) a second moiety that stably associates with said substrate surface;
wherein said contacting occurs under conditions sufficient to stably associate said polyectrolyte layer to said substrate surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, 16, 18, 19)
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12. A method for covalently bonding a polyelectrolyte layer to a substrate surface, comprising:
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(a) providing a substrate having a polyelectrolyte layer deposited on said surface thereof;
(b) contacting said polyelectrolyte layer and said substrate surface with an agent having a passivating group capable of covalently bonding to said polyelectrolyte layer and an anchoring group capable of covalently bonding to said substrate surface; and
(c) subjecting said substrate surface and said polyelectrolyte layer to a condition(s) that causes said passivating group to covalently bond to said polyelectrolyte layer and said anchoring group to covalently bond to said substrate surface.
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17. A method for fabricating a microarray, said method comprising:
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(a) producing a polyelectrolyte layer on at least one surface of a substrate;
(b) depositing a plurality of spots of ligands on said polyelectrolyte layer, said ligand spots arranged in an array pattern;
(c) contacting said polyelectrolyte layer with a reagent comprising;
(i) a passivating moiety that reacts with said polyelectrolyte layer, and (ii) a substrate reactive functionality; and
(d) producing a covalent bond between said substrate reactive functionality and said substrate.
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20. A method for fabricating a microarray, said method comprising:
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(a) producing a polyamine layer on at least one silica surface of a substrate;
(b) depositing a plurality of spots of ligands on said polyamine layer, said ligand spots arranged in an array pattern;
(c) contacting said polyamine layer with a solution of a trialkoxysilyl anhydride; and
(d) forming a covalent bond between said anhydride and said polyamine layer and a covalent silicon-oxygen bond between said trialkoxysilyl propyl anhydride and said silica surface on said substrate. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 29, 30, 31, 32, 33, 34, 35, 36, 37, 39, 40)
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28. A microarray comprising:
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(a) a polyelectrolyte layer stably associated with a substrate surface; and
(b) a plurality of ligand spots on said polyelectrolyte layer. - View Dependent Claims (38)
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Specification