Simultaneous handling of magnetic beads in a two-dimensional arrangement

• US 20030012699A1
• Filed: 06/13/2002
• Published: 01/16/2003
• Est. Priority Date: 11/18/1998
• Status: Abandoned Application

• Current Assignee: Cybio Instruments GmbH (Endress+Hauser AG)
• Original Assignee: Cybio Instruments GmbH (Endress+Hauser AG)
• Inventors: Moore, Thomas, Zimmermann, Peter
• Application Number:
  US10/172,560
• Publication Number:
  US 20030012699A1
• Time in Patent Office: Days
• US Class Current: 422/100
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates
• CPC Class Codes: G01N 35/1074 arranged in a two-dimensional array

Abstract

An arrangement for handling and selective magnetic filed directed horizontally magnetic rinsing of particles and liquids, magnetic beads and vessels, comprises a two-dimensional pipette arrangement, a two-dimensional magnet holder arrangement and a two-dimensional arrangement of cavities. The arrangements are in combination with a handling mechanism.

Claims

1. An arrangement for handling liquids, magnetic beads and vessels, comprising:

a two-dimensional pipette arrangement for pipetting liquid from a two-dimensional arrangement of cavities;

a two-dimensional magnet holder arrangement arranged proximate to the two-dimensional pipette arrangement and the two-dimensional arrangement of cavities; and

said arrangements in combination with handling mechanism for a horizontal movement connected to at least one of said arrangements to alternatively direct a magnetic field into a predetermined side of pipettes arranged in the pipette arrangement or a predetermined side of the cavities to provide a controllable horizontal resultant rinsing motion for particles in the liquid.

2. The arrangement according to claim 1, wherein the two-dimensional pipette arrangement is a simultaneously operating automatic pipetting/metering appliance with at least four tips/needles.

3. The arrangement according to claim 1, wherein the two-dimensional magnet holder arrangement is a carrier plate for a multiplicity of permanent magnets which are arranged in the form of a geometric matrix.

4. The arrangement according to claim 1, wherein the two-dimensional magnet holder arrangement is a carrier plate for a multiplicity of permanent-magnet bars which are arranged in rows.

5. The arrangement according to claim 1, wherein the two-dimensional magnet holder arrangement is a carrier plate for a multiplicity of permanent-magnet bars which are arranged in columns.

6. The arrangement according to claim 1, wherein the two-dimensional magnet holder arrangement is a comb-like arrangement of permanent-magnet bars.

7. The arrangement according to claim 1, wherein the two-dimensional arrangement of cavities is oriented to the microtitration plate format.

8. The arrangement according to claim 2, wherein the geometric arrangement of the tips/needles follows a standard microtitration plate format.

9. The arrangement according to claim 3, wherein the arrangement of the permanent magnets is oriented to the cavities of the microtitration plate.

10. The arrangement according to claim 6, wherein the grid of the comb arrangement results from the grid of tips of the simultaneous metering appliance, so that the magnet bars fit into the spaces between the tips.