Detection of analytes in aqueous environments

• US 20030013204A1
• Filed: 07/12/2002
• Published: 01/16/2003
• Est. Priority Date: 08/04/2000
• Status: Abandoned Application

• Current Assignee: Arthur E. Colvin, Jr.
• Original Assignee: Arthur E. Colvin, Jr.
• Inventors: Colvin, Arthur E. Jr.
• Application Number:
  US10/193,245
• Publication Number:
  US 20030013204A1
• Time in Patent Office: Days
• US Class Current: 436/166
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1459 invasive, e.g. introduced into the body by a catheter A61B5/1464 takes precedence
• CPC Class Codes: C07D 257/02 not condensed with other rings
• CPC Class Codes: G01N 2021/6432 Quenching
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/144444 Glucose

Abstract

The invention relates to indicator molecules for detecting the presence or concentration of an analyte in a medium, such as a liquid, and to methods for achieving such detection. More particularly, the invention relates to copolymer macromolecules containing relatively hydrophobic indicator component monomers, and hydrophilic monomers, such that the macromolecule is capable of use in an aqueous environment.

Claims

1. A method for the production of an indicator macromolecule for detecting the presence or concentration of an analyte in an aqueous environment, said method comprising copolymerizing:

a) one or more indicator component monomers which individually are not sufficiently water soluble to permit their use in an aqueous environment for detecting the presence or concentration of said analyte; and

b) one or more hydrophilic monomers;

such that the resulting macromolecule is capable of detecting the presence or concentration of said analyte in an aqueous environment.

2. The method of claim 1, wherein the indicator component monomer comprises an N-(o-boronobenzyl)amino]methyl]anthracene derivative.

3. The method of claim 1, wherein the hydrophilic monomer comprises [3-(methacryloylamino)-propyl]trimethylammonium chloride.

4. The method of claim 1, wherein the indicator component monomer is selected from the group consisting of a lanthanide chelate and a polyaromatic hydrocarbon.

5. The method of claim 1, wherein the molar ratio of hydrophilic monomer:

indicator component momomer is from about 2;

1 to about 500;

1.

6. The method of claim 5, wherein the ratio of hydrophilic monomer:

indicator component momomer is from about 5;

1 to about 50;

1.

7. The method of claim 6, wherein the ratio of hydrophilic monomer:

indicator component momomer is about 5;

1.

8. The method of claim 1, wherein the analyte detected is selected from the group consisting of a cis-diol;

oxygen;

carbon dioxide; and

zinc, potassium, hydrogen, or carbonate ions.

9. The method of claim 8, wherein the analyte detected is a saccharide.

10. The method of claim 9, wherein the saccharide is glucose.

11. The method of claim 1, wherein

i) the molar ratio of hydrophilic monomer:

indicator component momomer is from about 2;

1 to about 15;

1,

ii) the indicator component monomer comprises an N-(o-boronobenzyl)amino]methyl]anthracene derivative,

iii) the hydrophilic monomer comprises [3-(methacryloylamino)propyl]trimethylammonium chloride, and

iv) the macromolecule exhibits an excimer effect.