Plant microsatellite markers and methods for their use
First Claim
Patent Images
1. An isolated polynucleotide comprising a sequence selected from the group consisting of:
- (a) sequences provided in SEQ ID NO;
1-1054; and
(b) sequences complementary to a sequence provided in SEQ ID NO;
1-1054;
(c) sequences having at least about a 99% probability of being the same as a sequence of (a) or (b) as measured using computer algorithm BLASTN;
(d) sequences having at least 75% identity to a sequence of (a) or (b); and
(e) sequences having at least 90% identity to a sequence of (a) or (b).
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Abstract
Microsatellite sequences and associated flanking sequences isolated from pine and eucalyptus are provided, together with methods for the use of such sequences in the detection of polymorphic genetic markers. Kits comprising oligonucleotide primers and/or hybridization probes for use in such methods are also provided.
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Citations
25 Claims
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1. An isolated polynucleotide comprising a sequence selected from the group consisting of:
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(a) sequences provided in SEQ ID NO;
1-1054; and
(b) sequences complementary to a sequence provided in SEQ ID NO;
1-1054;
(c) sequences having at least about a 99% probability of being the same as a sequence of (a) or (b) as measured using computer algorithm BLASTN;
(d) sequences having at least 75% identity to a sequence of (a) or (b); and
(e) sequences having at least 90% identity to a sequence of (a) or (b). - View Dependent Claims (24)
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2. An isolated polynucleotide comprising a sequence selected from the group consisting of:
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(a) left flanking sequences of DNA sequences provided in SEQ ID NO;
1-1054;
(b) sequences complementary to a sequence of (a); and
(c) sequences having at least a 99% probability of being the same as a sequence of (a) or (b);
(d) sequences having at least 75% identity to a sequence of (a) or (b); and
(e) sequences having at least 90% identity to a sequence of (a) or (b).
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3. An isolated polynucleotide comprising a sequence selected from the group consisting of:
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(a) right flanking sequences of DNA sequences provided in SEQ ID NO;
1-1054;
(b) sequences complementary to a sequence of (a); and
(c) sequences having at least a 99% probability of being the same as a sequence of (a) or (b);
(d) sequences having at least 75% identity to a sequence of (a) or (b); and
(e) sequences having at least 90% identity to a sequence of (a) or (b).
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4. An isolated polynucleotide comprising a sequence selected from the group consisting of:
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(a) at least three contiguous repeats of a sequence provided in SEQ ID NO;
1055;
(b) at least three contiguous repeats of a sequence provided in SEQ ID NO;
1056;
(c) at least three contiguous repeats of a sequence provided in SEQ ID NO;
1057;
(d) sequences complementary to a sequence of (a), (b) or (c); and
(e) sequences having at least a 99% probability of being the same as a sequence of (a), (b) or (c);
(f) sequences having at least 75% identity to a sequence of (a), (b) or (c); and
(g) sequences having at least 90% identity to a sequence of (a), (b) or (c).
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5. An oligonucleotide primer that binds specifically to an isolated polynucleotide selected from the group consisting of SEQ ID NO:
- 1-1054, wherein the oligonucleotide primer comprises at least 6 contiguous nucleotides of a sequence complementary to a sequence provided in SEQ ID NO;
1-1054. - View Dependent Claims (25)
- 1-1054, wherein the oligonucleotide primer comprises at least 6 contiguous nucleotides of a sequence complementary to a sequence provided in SEQ ID NO;
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6. An oligonucleotide primer that binds specifically to an isolated polynucleotide selected from the group consisting of SEQ ID NO:
- 1-1054, wherein the oligonucleotide primer comprises at least 10 contiguous nucleotides of a sequence complementary to a sequence provided in SEQ ID NO;
1-1054.
- 1-1054, wherein the oligonucleotide primer comprises at least 10 contiguous nucleotides of a sequence complementary to a sequence provided in SEQ ID NO;
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7. An oligonucleotide primer that binds specifically to an isolated polynucleotide selected from the group consisting of SEQ ID NO:
- 1-1054, wherein the oligonucleotide primer comprises at least 20 contiguous nucleotides of a sequence complementary to a sequence provided in SEQ ID NO;
1-1054.
- 1-1054, wherein the oligonucleotide primer comprises at least 20 contiguous nucleotides of a sequence complementary to a sequence provided in SEQ ID NO;
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8. An isolated oligonucleotide primer pair selected from the group consisting of:
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(a) at least 10 contiguous nucleotides of a left flanking sequence provided in Table 1 and at least 10 contiguous nucleotides of a right flanking sequence provided in Table 2, wherein the left flanking sequence and right flanking sequence are associated with the same SEQ ID NO;
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(b) a sequence pair complementary to a sequence pair of (a); and
(c) a sequence pair having at least 90% identity to a sequence pair of (a) or (b). - View Dependent Claims (9)
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10. A method for detecting a polymorphic genetic marker in a subject, comprising:
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(a) isolating DNA from the subject; and
(b) analyzing the isolated DNA for the presence of the polymorphic genetic marker using at least one oligonucleotide to detect the polymorphic marker, wherein the oligonucleotide comprises at least 6 contiguous residues of a sequence selected from the group consisting of;
(i) sequences provided in SEQ ID NO;
1-1054;
(ii) sequences complementary to a sequence of SEQ ID NO;
1-1054; and
(iii) sequences having at least 90% identity to a sequence of (i) or (ii). - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 19, 20, 21, 22, 23)
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18. A method for detecting a polymorphic genetic marker in a subject comprising:
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(a) isolating DNA from the subject;
(b) contacting the isolated DNA with an oligonucleotide probe in a hybridization assay to detect the presence of DNA molecules that hybridize to the oligonucleotide probe, wherein the oligonucleotide probe is specific for a polynucleotide having a sequence selected from the group consisting of;
(i) sequences provided in SEQ ID NO;
1-1054;
(ii) sequences complementary to a sequence of SEQ ID NO;
1-1054; and
(iii) sequences having at least 90% identity to a sequence of (i) or (ii);
(c) separating the DNA molecules according to size; and
(d) analyzing the DNA molecules for the presence of the polymorphic genetic marker.
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Specification