Osteoblast precursors from human embryonic stem cells
First Claim
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1. A set of two isolated cell populations for generating human osteoblast lineage cells, consisting of:
- a first cell population comprising pluripotent stem (pPS) cells isolated from a human blastocyst, and a second cell population that proliferates in culture, comprising at least ˜
30% pPS derived osteoblasts or osteoblast precursors, identifiable by the criteria that they are progeny of said pPS cells, and have at least one of the following characteristics;
they express osteonectin they express osteocalcin they form an extracellular matrix comprising calcium when cultured in vitro.
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Abstract
This invention provides populations of mesenchymal cells obtained from pluripotent stem cells by differentiating them ex vivo. Multipotent mesenchymal cells can in turn be differentiated into more specialized cell types such as osteoblasts, with properties that make them suitable for reconstituting musculoskeletal cell function in an individual. The compositions, methods, and techniques described in this disclosure can be used for a variety of commercially important diagnostic, drug screening, and therapeutic applications.
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12 Claims
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1. A set of two isolated cell populations for generating human osteoblast lineage cells, consisting of:
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a first cell population comprising pluripotent stem (pPS) cells isolated from a human blastocyst, and a second cell population that proliferates in culture, comprising at least ˜
30% pPS derived osteoblasts or osteoblast precursors, identifiable by the criteria that they are progeny of said pPS cells, and have at least one of the following characteristics;
they express osteonectin they express osteocalcin they form an extracellular matrix comprising calcium when cultured in vitro. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification