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Use of site-specific nicking endonucleases to create single-stranded regions and applications thereof

  • US 20030022317A1
  • Filed: 12/15/2000
  • Published: 01/30/2003
  • Est. Priority Date: 12/15/2000
  • Status: Active Grant
First Claim
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1. A method for creating a target single-stranded region in double-stranded DNA comprising:

  • (a) nicking at least one site bordering the target region in double-stranded DNA with at least one site-specific nicking endonuclease; and

    (b) subjecting the nicked DNA to conditions where the target region is selectively denatured.

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