Cardiomyocyte precursors from human embryonic stem cells
First Claim
1. A set of two isolated cell populations for generating human cells of the cardiomyocyte lineage, consisting of:
- a first cell population comprising pluripotent stem (pPS) cells isolated from a human blastocyst, and a second cell population that proliferates in culture, comprising at least ˜
5% pPS derived cardiomyocyte and cardiomyocyte precursors, identifiable by the criteria that they are progeny of said pPS cells, and either have spontaneous contractile activity, or express at least one of the following markers from an endogenous gene;
cardiac troponin I (cTnI), cardiac troponin T (cTnT), or atrial natriuretic factor (ANF).
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Abstract
This invention provides populations human cells of the cardiomyocyte lineage. The cells are obtained by causing cultures of pluripotent stem cells to differentiate in vitro, and then harvesting cells with certain phenotypic features. Differentiated cells bear cell surface and morphologic markers characteristic of cardiomyocytes, and a proportion of them undergo spontaneous periodic contraction. Highly enriched populations of cardiomyocytes and their replicating precursors can be obtained, suitable for use in a variety of applications, such as drug screening and therapy for cardiac disease.
45 Citations
15 Claims
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1. A set of two isolated cell populations for generating human cells of the cardiomyocyte lineage, consisting of:
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a first cell population comprising pluripotent stem (pPS) cells isolated from a human blastocyst, and a second cell population that proliferates in culture, comprising at least ˜
5% pPS derived cardiomyocyte and cardiomyocyte precursors, identifiable by the criteria that they are progeny of said pPS cells, and either have spontaneous contractile activity, or express at least one of the following markers from an endogenous gene;
cardiac troponin I (cTnI), cardiac troponin T (cTnT), or atrial natriuretic factor (ANF). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification