Method of making a plastic colorimetric resonant biosensor device with liquid handling capabilities

• US 20030026891A1
• Filed: 07/23/2002
• Published: 02/06/2003
• Est. Priority Date: 10/30/2000
• Status: Active Grant

• Current Assignee: X-Body Incorporated (Bristol-Myers Squibb Company)
• Original Assignee: Sru Biosystems Incorporated
• Inventors: Li, Peter, Cunningham, Brian, Qiu, Jean
• Granted Patent: US 7,175,980 B2
• Time in Patent Office: Days
• US Class Current: 427/58
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/4788 Diffraction for sizing particles G01N15/0205
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G02B 21/34 Microscope slides, e.g. mounting specimens on microscope slides
• CPC Class Codes: G02B 5/1809 with pitch less than or comparable to the wavelength
• CPC Class Codes: Y10T 156/10 Methods of surface bonding and/or assembly therefor

Abstract

Methods of producing liquid handling biosensor devices are provided. The liquid handling biosensor devices allow detection of biomolecular interactions in liquid. The use of labels is not required and the methods can be performed in a high-throughput manner.

Claims

1. A method of making a liquid handling colorimetric resonant reflection biosensor device, comprising:

a. applying an attachment material to a surface of a transfer block,

b. contacting a liquid holding part with the attachment material on the surface of the transfer block,

c. removing the liquid holding part and any attachment material present on the liquid holding part from the transfer block,

d. contacting a biosensor with the liquid holding part so that the attachment material is between the biosensor and the liquid holding part, and

e. exposing the liquid holding part and biosensor to ultraviolet light to solidify the attachment material, wherein the liquid holding part is immobilized onto the biosensor.

2. The method of claim 1, wherein the biosensor comprises a grating comprised of or coated with a dielectric material having a high refractive index or a reflective material, wherein when the biosensor is illuminated a resonant grating effect is produced on the reflected radiation spectrum.

3. The method of claim 1, wherein the attachment material is an ultraviolet (UV) curable polymer.

4. The method of claim 1, wherein the liquid holding part is a bottomless microtiter plate or a fluid flow channel.

5. The method of claim 1, wherein the attachment material is applied by a knife coating technique or a rod coating technique.

6. The method of claim 1, wherein the step of contacting further comprises placing the biosensor in a first part of a holding fixture and placing the liquid handling part in a second part of the holding fixture, wherein the first and second parts of the holding fixture are brought together so that the biosensor and the liquid holding part are in contact with each other.

7. The method of claim 1, wherein the thickness of the attachment material on the surface of the transfer block is about 0.025 mm to about 5 mm thick.

8. A method of making a liquid handling colorimetric resonant reflection biosensor device, comprising:

(a) die cutting a transfer pressure sensitive adhesive into a pattern of a liquid holding part to create a patterned adhesive;

(b) transferring the patterned adhesive onto the liquid holding part;

(c) contacting a biosensor with the patterned adhesive of the liquid holding part. and

(d) applying pressure to the biosensor or liquid holding part;

whereby a liquid handling colorimetric resonant reflection biosensor device is made.

9. The method of claim 8, wherein the biosensor comprises a grating comprised of or coated with a dielectric material having a high refractive index or a reflective material, wherein when the biosensor is illuminated a resonant grating effect is produced on the reflected radiation spectrum.

10. The method of claim 8, wherein the liquid holding part is a bottomless microtiter plate or a fluid flow channel.

11. A method of making a liquid handling calorimetric resonant reflection biosensor device comprising laser welding or ultrasonic welding a liquid holding part to a calorimetric resonant reflection biosensor.

12. The method of claim 11, wherein the biosensor comprises a grating comprised of or coated with a dielectric material having a high refractive index or a reflective material, wherein when the biosensor is illuminated a resonant grating effect is produced on the reflected radiation spectrum.

13. The method of claim 11, wherein the liquid holding part is a bottomless microtiter plate or a fluid flow channel.