×

Method of haplotyping and kit therefor

  • US 20030027150A1
  • Filed: 08/03/2001
  • Published: 02/06/2003
  • Est. Priority Date: 08/03/2001
  • Status: Abandoned Application
First Claim
Patent Images

1. A method of identifying the haplotype of an organism, the method comprising:

  • (a) providing a sample comprising nucleic acids from the organism, wherein the nucleic acids comprise at least two copies of an isogenic nucleotide sequence of interest, (b) aliquotting the nucleic acids into test locations such that at least one test location is expected to contain one, and only one, isogenic nucleotide sequence of interest, (c) amplifying the isogenic nucleotide sequence of interest in a predetermined number of test locations to create amplification products, (i) wherein amplifying the isogenic nucleotide sequence of interest employs two pairs of oligonucleotide primers (ii) such that at least one test location is expected to contain amplification products having a unique nucleotide sequence corresponding to the nucleotide sequence of one, and only one, of the isogenic nucleotide sequences of interest in the organism'"'"'s genome, and (d) detecting the presence or absence of specific forms of a first nucleotide polymorphism and a second nucleotide polymorphism in the isogenic region of interest at two non-contiguous positions in the nucleotide sequence of interest by detecting the presence or absence of specific forms of the first nucleotide polymorphism and second nucleotide polymorphism in the amplification products in each of the predetermined number of test locations comprising amplified nucleic acids, such that the haplotype of the organism is identified.

View all claims
  • 0 Assignments
Timeline View
Assignment View
    ×
    ×