Innate immunity markers for rapid diagnosis of infectious diseases
First Claim
1. A method for determining the type of an infectious pathogen in a patient who is suspected to be suffering from an infectious pathogen, comprising:
- a) measuring the amounts of each of a plurality of markers in a specimen obtained from the patient, wherein each of the markers is produced by the patient as a part of that patient'"'"'s innate immune response to the presence of the infectious pathogen and the plurality of markers is indicative of the type of the infectious pathogen;
b) identifying a marker profile based on the measured amounts of each of the plurality of markers; and
c) if the marker profile is indicative of an infection, then determining the type of infectious pathogen from the marker profile.
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Accused Products
Abstract
A method is provided for determining the type of an infectious pathogen in a patient who is suspected to be suffering from an infectious pathogen. The method involves first measuring the amounts of a plurality of markers in a body fluid sample of the patient. The markers of interest are produced by the patient as part of that patient'"'"'s innate immune response to the presence of the infectious pathogen and are indicative of the type of the infectious pathogen in the patient. Next, a marker profile is identified based on the measured amounts of the plurality of markers. Finally, if the marker profile is indicative of an infection, then the type of infectious pathogen within the patient is determined from the marker profile. In preferred embodiments, any individual marker is either an mRNA or a protein. Methods for identifying suitable markers and kits are provided as well.
23 Citations
43 Claims
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1. A method for determining the type of an infectious pathogen in a patient who is suspected to be suffering from an infectious pathogen, comprising:
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a) measuring the amounts of each of a plurality of markers in a specimen obtained from the patient, wherein each of the markers is produced by the patient as a part of that patient'"'"'s innate immune response to the presence of the infectious pathogen and the plurality of markers is indicative of the type of the infectious pathogen;
b) identifying a marker profile based on the measured amounts of each of the plurality of markers; and
c) if the marker profile is indicative of an infection, then determining the type of infectious pathogen from the marker profile. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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27. An assay kit for determining the presence of an infectious pathogen in a patient, comprising:
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a) a plurality of biomolecular probes each complementary to a different marker within a plurality of markers, such that one or more probe-marker complexes is formed under binding conditions, is at least partially indicative of the presence of an infectious pathogen;
b) a plurality of label probes each having a region that binds directly or indirectly to one or more probe-marker complexes; and
c) written instructions for carrying out the assay. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36)
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37. A method for identifying a marker that is indicative of the presence of an infectious pathogen in a patient, comprising:
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comparing (a) the genome-wide expression of genes of a specimen obtained from a patient who is infected with the infectious pathogen to (b) the genome-wide expression of genes of a specimen obtained from an individual who is not infected with the infectious pathogen, wherein a gene expressed in (a) and not in (b) indicates a gene associated with the presence of the infectious pathogen; and
determining from the gene associated with the presence of an infectious pathogen, the corresponding marker. - View Dependent Claims (38, 39, 40)
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41. A method for identifying a protein marker that is indicative of the presence of an infectious pathogen in a patient, comprising:
comparing (a) the proteins present in a specimen obtained from a patient who is infected with the infectious pathogen to (b) the proteins present in a specimen obtained from an individual who is not infected with the infectious pathogen, wherein a protein present in (a) and not in (b) represents a protein marker that is indicative of the presence of an infectious pathogen. - View Dependent Claims (42, 43)
Specification