Tissue engineered heart valve
First Claim
Patent Images
1. A human engineered tissue-type heart valve comprising:
- a plurality of leaflets assembled to form a heart valve, wherein each leaflet is comprised of at least five layers of at least one living tissue sheet fused together to form a self-supporting human engineered tissue.
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Abstract
The tissue-engineered heart valve of the present invention is comprised of elements, such as leaflets, formed from self-supporting human engineered tissue. Such self-supporting tissue is comprised of living biological cells and extracellular matrix without the presence of nonviable scaffolding structures. Thus, the tissue-engineered heart valve of the present invention consists of totally living human tissue which could theoretically function like a native biological structure with the potential to grow, to repair and to remodel.
271 Citations
55 Claims
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1. A human engineered tissue-type heart valve comprising:
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a plurality of leaflets assembled to form a heart valve, wherein each leaflet is comprised of at least five layers of at least one living tissue sheet fused together to form a self-supporting human engineered tissue. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A human engineered tissue-type heart valve comprising:
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a plurality of leaflets assembled to form a heart valve, wherein each leaflet is comprised of layers of at least one living tissue sheet fused together to form a self-supporting human engineered tissue having a thickness of at least approximately 0.16 mm.
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17. A human engineered tissue-type heart valve comprising:
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a plurality of leaflets arranged to form a heart valve, wherein each leaflet is comprised of layers of at least one allogeneic living tissue sheet fused together to form a self-supporting human engineered tissue which undergoes living cell replacement upon implantation in a patient so that at least some of the allogeneic cells are replaced with the patient'"'"'s living cells. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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28. A method of making a human engineered heart valve, the method comprising:
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generating at least one living tissue sheet by secreting an extracellular matrix from cells;
layering the at least one living tissue sheet to form a layered construct having at least seven layers; and
culturing the layered construct to fuse the layers to form a human engineered tissue. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35, 36, 37)
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38. A method of preparing human engineered tissue for use in making a heart valve, the method comprising:
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generating at least one living tissue sheet by secreting an extracellular matrix from cells;
layering the at least one living tissue sheet to form a layered construct;
culturing the layered construct to fuse the layers to form the human engineered tissue; and
regulating shrinkage of the human engineered tissue. - View Dependent Claims (39, 40, 41, 42, 43, 44, 45)
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46. A method of preparing human engineered tissue for use in making a heart valve, the method comprising:
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generating at least one living tissue sheet by secreting an extracellular matrix from cells;
layering the at least one living tissue sheet to form a layered construct;
culturing the layered construct to fuse the layers to form the human engineered tissue; and
cutting a leaflet shape out of the human engineered tissue which is dimensionally larger than a desired leaflet shape to account for shrinkage. - View Dependent Claims (47, 48, 49, 50)
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51. A human engineered tissue-type heart valve comprising:
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a tissue leaflet subassembly mated with a wireform to form a heart valve, wherein each leaflet is comprised of at least five layers of at least one living tissue sheet fused together to form a self-supporting human engineered tissue, and wherein at least a portion of the wireform is covered with the tissue. - View Dependent Claims (52, 53, 54, 55)
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Specification