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Optical projection imaging system and method for automatically detecting cells with molecular marker compartmentalization associated with malignancy and disease

  • US 20030031352A1
  • Filed: 05/14/2002
  • Published: 02/13/2003
  • Est. Priority Date: 08/10/2001
  • Status: Active Grant
First Claim
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19. A method for the detection of cells with molecular marker compartmentalization associated with malignancy and disease including a cancer, the method comprising the steps of:

  • a) obtaining a cell sample having a plurality of cells and suspending the plurality of cells in a solution;

    b) if required, fixing the plurality of cells of the cell sample;

    c) labeling at least one molecular marker in the plurality of cells with tagged molecular probes or stains;

    d) illuminating at least one cell of the plurality of cells with at least one point source of light;

    e) obtaining at least one projection image through the at least one cell with a digital array detector;

    f) compensating the at least one projection image for variations in illumination;

    g) analyzing the at least one projection image to detect at least one labeled molecular marker;

    h) analyzing the location of the at least one molecular marker in two-dimensional (2D) space within the at least one cell;

    i) measuring compartmentalization of the at least one labeled molecular marker by using the densitometric and location features; and

    j) analyzing compartmentalization of the at least one labeled molecular marker with at least one classifier for the detection of malignancy and/or disease associated molecular marker-compartmentalization.

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