Low ionic strength method and composition for reducing bacterial attachment to biomaterials

• US 20030031587A1
• Filed: 05/15/2001
• Published: 02/13/2003
• Est. Priority Date: 05/15/2001
• Status: Active Grant

• Current Assignee: Bausch & Lomb Incorporated (Bausch Health Cos., Inc.)
• Original Assignee: Bausch & Lomb Incorporated (Bausch Health Cos., Inc.)
• Inventors: Salamone, Joseph C., Hu, Zhenze, Borazjani, Roya
• Granted Patent: US 6,702,983 B2
• Time in Patent Office: Days
• US Class Current: 422/28
• CPC Class Codes: A61L 2300/232 Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
• CPC Class Codes: A61L 2300/404 Biocides, antimicrobial agents, antiseptic agents
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61L 29/16 Biologically active materials, e.g. therapeutic substances A61L29/048 takes precedence
• CPC Class Codes: A61L 31/10 Macromolecular materials
• CPC Class Codes: C08L 1/02 Cellulose; Modified cellulose
• CPC Class Codes: G02B 1/043 Contact lenses

Abstract

A method is disclosed for inhibiting adhesion of bacteria to the surface of a biomedical device comprising contacting the surface of said biomedical device with a cationic polysaccharide in aqueous solution having an ionic strength of from about 0.01 to about 0.13.

Claims

1. A method for inhibiting adhesion of bacteria to the surface of a biomedical device comprising contacting the surface of said biomedical device with a cationic polysaccharide in aqueous solution having an ionic strength of from about 0.01 to about 0.13.

2. The method of claim 1 wherein said aqueous solution has an ionic strength of from about 0.05 to about 0.1.

3. The method of claim 1 wherein said aqueous solution has an ionic strength of less than about 0.095.

4. The method of claim 3 wherein said aqueous solution has an ionic strength of less than about 0.090.

5. The method of claim 1 further comprising treating the surface of said biomedical device to provide a net anionic charge on said surface before contacting said surface with said cationic polysaccharide.

6. The method of claim 1 wherein the surface of said biomedical device carries a net anionic surface charge and wherein the method includes no intermediate treatment step to modify the surface charge before binding said polysaccharide to the surface of said biomedical device.

7. The method of claim 5 wherein said surface treating step further comprises contacting said surface with a linking agent.

8. The method of claim 1 wherein said contacting step further comprises retaining said cationic polysaccharide on the surface of said biomedical device through at least one selected from the group consisting of ionic interactions, hydrogen-bonded interactions, hydrophobic interactions and covalent interactions.

9. The method of claim 8 wherein said ionic interactions are between oppositely charged ionic groups between the biomedical device and an aqueous solutions containing the cationic polysaccharide.

10. The method of claim 9 wherein the negative charge on the biomedical device is derived from at least one selected from the group consisting of carboxylate groups, sulfonate groups, phosphate groups and phosphonate groups.

11. The method of claim 9 wherein the cationic charge on the cationic polysaccharide is derived from quaternary ammonium groups, sulfonium groups, phosphonium groups, and other positively charged functional groups.

12. The method of claim 8 wherein said hydrogen-bonding interactions occur between hydrogen-bond accepting surfaces and hydrogen-bond donating solutions, or through hydrogen-bond donating surfaces and hydrogen-bond accepting surfaces.

13. The method of claim 12 wherein said hydrogen-bond accepting groups are selected from the group consisting of pyrrolidone groups, N,N-disubstituted acrylamide groups and polyether groups.

14. The method of claim 13 wherein said polyether groups are poly(ethylene glycol) or poly(ethylene oxide).

15. The method of claim 12 wherein said hydrogen-donating groups are selected from the group consisting of carboxylic acids, phosphoric acids, phosphonic acids and phenolic groups.

16. The method of claim 8 wherein said hydrophobic interactions occur through hydrophobic sites on the biomaterial surface interacting with hydrophobic groups on the cationic polysaccharide.

17. The method of claim 7 wherein said covalent interactions exist between the biomaterials surface and the water-soluble cationic polysaccharide such that the cationic polysaccharide is bound to the biomaterial surface.

18. The method of claim 1 wherein said biomedical device is an ophthalmic lens.

19. The method of claim 15 wherein said ophthalmic lens is selected from the group consisting of contact lenses, anterior chamber lenses, posterior chamber lenses intraocular lenses and corneal inlays.

20. The method of claim 1 wherein said biomedical device is a silicone hydrogel material.

21. The method of claim 19 wherein said contact lens is an extended-wear contact lens suitable for periods of continuous wear for about 7 to about 30 days.

22. The method of claim 1 wherein the cationic polysaccharide is selected from the group consisting of cationic starch, cationic dextran, cationic chitosan, cationic locust bean gum, cationic gum tragacanth, cationic curdlan, cationic pullulan and cationic scleroglucan.

23. The method of claim 1 wherein the biomedical device is selected from the group consisting of intraocular lenses, corneal inlays, contact lenses, contact lens cases, stents, implants and cathaters.