Cell isolation and screening device and method of using same
First Claim
1. A method of screening cells for a desired biological activity comprising:
- providing a cell isolation device defining a plurality of cell isolation regions, each cell isolation region encompassing a recess, each of the cell isolation regions being sized to isolate about one to about five cells therein, and the cell isolation regions further defining a predetermined pitch with respect to one another;
delivering the about one cell to about five cells to each of the cell isolation regions;
providing a cell expansion device defining a plurality of wells corresponding to respective ones of the plurality of cell isolation regions, the wells defining a predetermined pitch matching the predetermined pitch of the cell isolation regions;
transferring the about one cell to about five cells from the cell isolation regions to the wells;
allowing the about one cell to about five cells to proliferate and exhibit a desired biological activity in the wells;
assaying the desired biological activity of the one cell to about five cells.
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides methods and devices for screening a single cell or a small group of cells for a desired biological activity. In particular, the present invention provides for delivering cell(s) to a plurality of cell isolation regions of a cell isolation device, transferring cell(s) to a plurality of wells of a cell expansion device and then detecting the potential desired biological activity of the cell(s). Each of the receptacles comprise a recess sized to isolate a single cell or small group of cells and each of the wells encompass a cavity that provides sufficient volume for cell proliferation.
101 Citations
22 Claims
-
1. A method of screening cells for a desired biological activity comprising:
-
providing a cell isolation device defining a plurality of cell isolation regions, each cell isolation region encompassing a recess, each of the cell isolation regions being sized to isolate about one to about five cells therein, and the cell isolation regions further defining a predetermined pitch with respect to one another;
delivering the about one cell to about five cells to each of the cell isolation regions;
providing a cell expansion device defining a plurality of wells corresponding to respective ones of the plurality of cell isolation regions, the wells defining a predetermined pitch matching the predetermined pitch of the cell isolation regions;
transferring the about one cell to about five cells from the cell isolation regions to the wells;
allowing the about one cell to about five cells to proliferate and exhibit a desired biological activity in the wells;
assaying the desired biological activity of the one cell to about five cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
-
-
12. A kit for screening cells for a desired biological activity, the kit comprising:
-
a cell isolation device defining a plurality of cell isolation regions, each cell isolation region encompassing a recess, each of the cell isolation regions being sized to isolate about one to about five cells therein, and the cell isolation regions further defining a predetermined pitch with respect to one another;
a cell expansion device defining a plurality of wells corresponding to respective ones of the plurality of cell isolation regions, and the wells defining a predetermined pitch matching the predetermined pitch of the cell isolation regions. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
-
Specification