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Cell isolation and screening device and method of using same

  • US 20030032002A1
  • Filed: 07/29/2002
  • Published: 02/13/2003
  • Est. Priority Date: 07/27/2001
  • Status: Active Grant
First Claim
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1. A method of screening cells for a desired biological activity comprising:

  • providing a cell isolation device defining a plurality of cell isolation regions, each cell isolation region encompassing a recess, each of the cell isolation regions being sized to isolate about one to about five cells therein, and the cell isolation regions further defining a predetermined pitch with respect to one another;

    delivering the about one cell to about five cells to each of the cell isolation regions;

    providing a cell expansion device defining a plurality of wells corresponding to respective ones of the plurality of cell isolation regions, the wells defining a predetermined pitch matching the predetermined pitch of the cell isolation regions;

    transferring the about one cell to about five cells from the cell isolation regions to the wells;

    allowing the about one cell to about five cells to proliferate and exhibit a desired biological activity in the wells;

    assaying the desired biological activity of the one cell to about five cells.

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