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In vitro capture of nucleic acids via modified oligonucleotides and magnetic beads

  • US 20030032028A1
  • Filed: 02/15/2002
  • Published: 02/13/2003
  • Est. Priority Date: 06/12/2001
  • Status: Abandoned Application
First Claim
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1. An in vitro method of capturing one or more target sequences, wherein the method comprises the steps of:

  • providing one or more modified, high stringency oligonucleotide conjugates (“

    MHSO”

    ) comprising a nucleotide sequence that is complementary to a target sequence and a linking molecule;

    incubating a sample of nucleic acids comprising the target sequence to be captured with the MHSO conjugate under non-denaturing conditions, thereby forming one or more hybridized duplexes, wherein each duplex comprises a target sequence portion and a modified oligonucleotide conjugate portion;

    contacting substantially all of the hybridized duplexes with a linking source, such that the linking molecule of each duplex that contacts the linking source forms a bond with the linking source; and

    separating substantially all of the hybridized duplexes from the sample of nucleic acids by extracting the linking source from the sample.

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