Method and apparatus for detecting biomolecular interactions
First Claim
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1. An apparatus for detecting biochemical interactions occurring on a surface of a biosensor, said apparatus comprising:
- a light source;
a first optical fiber coupled to said light source, said first optical fiber illuminating said biosensor, a second optical fiber that detects a signal reflected from said surface of said biosensor; and
a spectrometer for determining spectra of said reflected signal from said biosensor.
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Abstract
Method and apparatus for detecting biomolecular interactions. The use of labels is not required and the methods may be performed in a high-throughput manner. An apparatus for detecting biochemical interactions occurring on the surface of a biosensor includes a light source. A first optical fiber is coupled to the light source and illuminates the biosensor. A second optical fiber detects a wavelength reflected from the biosensor. A spectrometer determines spectra of a reflected signal from the biosensor.
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Citations
33 Claims
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1. An apparatus for detecting biochemical interactions occurring on a surface of a biosensor, said apparatus comprising:
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a light source;
a first optical fiber coupled to said light source, said first optical fiber illuminating said biosensor, a second optical fiber that detects a signal reflected from said surface of said biosensor; and
a spectrometer for determining spectra of said reflected signal from said biosensor. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A biosensor imaging system comprising:
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a light source generating white light;
a collimator for collimating said white light onto a surface of a biosensor;
an imaging spectrometer receiving reflected light from said biosensor, and software calculating a PWV of said reflected light. - View Dependent Claims (11, 12, 13, 14, 15, 16)
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17. An instrument for detecting light reflected from a surface of a biosensor, said instrument comprising:
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a light source;
an illuminating fiber optically coupled to said light source, said illuminating fiber illuminating an area of said surface of said biosensor, a detecting fiber for detecting radiation reflected from said surface;
a spectrometer receiving said reflected radiation; and
software determining a peak wavelength value of said reflected wavelength. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24)
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25. A method of measuring biochemical surface interactions of a biosensor, said method comprising the steps of:
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incubating said biosensor at a predetermined temperature;
providing a dual fiber optic probe with at least one illuminating fiber that is optically coupled to a light source, said illuminating fiber directly illuminating light towards a reflective surface of said biosensor;
providing at least one detecting fiber, said at least one detecting fiber detecting light reflected from said reflective surface of the biosensor;
optically coupling said at least one detecting fiber to a spectrometer, and processing said reflected light from said reflective surface of said biosensor. - View Dependent Claims (26, 27, 28, 29, 30, 31)
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32. An instrument for detecting a reflected wavelength, comprising:
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a light source for generating a collimated quantity of white light;
an illuminating fiber optically coupled to said light source, said illuminating fiber lluminating an illumination area of a surface of an optical device;
a detecting fiber for detecting a reflected wavelength, said wavelength reflected from said surface of said optical device; and
a single-point spectrometer that processes said reflected wavelength and derives a peak wavelength value. - View Dependent Claims (33)
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Specification