Peelable and resealable devices for biochemical assays

• US 20030032046A1
• Filed: 07/29/2002
• Published: 02/13/2003
• Est. Priority Date: 11/08/2000
• Status: Active Grant

• Current Assignee: Surface Logix, Inc.
• Original Assignee: Surface Logix, Inc.
• Inventors: Kirk, Gregory L., Campbell, Stewart, Duffy, David, Schueller, Olivier, Agosto, Melina
• Granted Patent: US 7,371,563 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01D 2325/08 Patterned membranes
• CPC Class Codes: B01J 2219/00274 Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00662 Two-dimensional arrays within two-dimensional arrays
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/00743 Cells
• CPC Class Codes: B01L 2200/021 Adjust spacings in an array of wells, pipettes or holders, format transfer between arrays of different size or geometry
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0848 Specific forms of parts of containers
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/123 Flexible; Elastomeric
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 5/00 : Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C12M 23/12 : Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12Q 1/025 : for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics antimicrobial activity C12Q1/18
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 15/1468 : with spatial resolution of the texture or inner structure of the particle
• CPC Class Codes: G01N 2015/1497 : Particle shape
• CPC Class Codes: G01N 33/54366 : Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G06T 2207/30072 : Microarray; Biochip, DNA array; Well plate
• CPC Class Codes: G06T 7/0012 : Biomedical image inspection
• CPC Class Codes: G06T 7/70 : Determining position or orientation of objects or cameras camera calibration G06T7/80
• CPC Class Codes: Y10T 428/24281 : Struck out portion type
• CPC Class Codes: Y10T 428/24289 : Embedded or interlocked
• CPC Class Codes: Y10T 428/24306 : Diamond or hexagonal

Abstract

Devices and methods for performing assays on materials, particularly biological materials, are provided. The devices and methods make use of self-sealing members, which can be applied to a flat surface to form wells to facilitate immobilization of materials on the flat surface, then removed to yield a flat surface that facilitates the performance of processes on and/or detection of the immobilized material.

Claims

1. A kit for forming a device comprising:

a base plate;

a first removable member adapted to repeatedly self-seal on the base plate, the first removable member defining at least one first orifice therein and having walls bounding the at least one first orifice on sides of the at least one first orifice, the first removable member further being configured such that, when it is placed in self-sealing contact with the base plate, it defines therewith at least one first well corresponding to a respective one of the at least one first orifice;

a second removable member adapted to repeatedly self-seal on at least one of the first removable member and the base plate, the second removable member defining the at least one second orifice therein and having walls bounding the at least one second orifice on sides of the at least one second orifice, the second removable member further being configured such that, when it is placed in self-sealing contact with one of the first removable member and the base plate, it defines therewith at least one second well corresponding to a respective one of the at least on first orifice, wherein the device is formed when the at least one first well and the at least one second well are defined by assembling selected ones of the base plate, the first removable member and the second removable member.

2. The kit of claim 1, wherein the first removable member and the second removable member are configured such that, when the first removable member is placed in self-sealing contact with the base plate, and the second removable member is placed in self-sealing contact with the first removable member, the at least one second well encompasses a respective one of the at least one first well.

3. The kit of claim 1, wherein the first removable member and the second removable member are configured such that, when the first removable member is placed in self-sealing contact with the base plate, and the second removable member is placed in self-sealing contact with the first removable member, the at least one second orifice exposes at least a portion of an upper surface of the first removable member.

4. The kit of claim 1, wherein the first removable member and the second removable member are configured such that, when the first removable member is placed in self-sealing contact with the base plate, and the second removable member is placed in self-sealing contact with the first removable member, the at least one second well encompasses a respective one of the at least one first well, and the at least one second orifice exposes at least a portion of an upper surface of the first removable member.

5. The kit of claim 1, wherein the walls of the first removable member bounding the at least one first orifice are tapered in a direction from an upper surface of the first removable member toward a lower surface of the first removable member such that an opening of the at least one first orifice is larger in the upper surface of the first removable member than an opening of the at least one first orifice in the lower surface of the first removable member.

6. The kit of claim 1, wherein the walls of the second removable member bounding the at least one second orifice are tapered in a direction from an upper surface of the second removable member toward a lower surface of the second removable member such that an opening of the at least one second orifice is larger in the upper surface of the second removable member than an opening of the at least one second orifice in the lower surface of the second removable member.

7. The kit of claim 5, wherein the walls of the second removable member bounding the at least one second orifice are tapered in a direction from an upper surface of the second removable member toward a lower surface of the second removable member such that an opening of the at least one second orifice is larger in the upper surface of the second removable member than an opening of the at least one second orifice in the lower surface of the second removable member, a degree of tapering of the walls of the first removable member being substantially equal to a degree of tapering of the walls of the second removable member.

8. The kit of claim 1, wherein a contour of an opening of the at least one second orifice in a lower surface of the second removable member is substantially identical to a contour of a corresponding opening of the at least one first orifice in an upper surface of the first removable member.

9. The kit of claim 8, wherein the first removable member and the second removable member are configured such that, when the first removable member is placed in self-sealing contact with the base plate, and the second removable member is placed in self-sealing contact with the first removable member, the opening of the at least one first orifice registers with the opening of the at least one second orifice such that the walls bounding said the at least one first orifice form a continuous surface with the walls bounding said the at least one second orifice.

10. The kit of claim 1, wherein at least one of the at least one first orifice and the at least one second orifice includes a plurality of orifices defining an array that spatially and dimensionally corresponds to a standard array of wells in an industry standard microtiter plate.

11. The kit of claim 8, wherein the standard microtiter plate is selected from the group consisting of a 6-well microtiter plate, a 12-well microtiter plate, a 24-well microtiter plate, a 96-well microtiter plate, a 384-well microtiter plate, a 1,536-well microtiter plate, and a 9,600-well microtiter plate.

12. The kit of claim 1, wherein the base plate is made of a material selected from the group consisting of glass, silicon, fused silica, metal films, polystyrene, poly(methylacrylate), and polycarbonate.

13. The kit of claim 1, further comprises a first layer of metal disposed on an upper surface of the base plate.

14. The kit of claim 13, wherein the first layer of metal made of a material is selected from the group consisting of gold, silver, platinum, palladium and copper.

15. The kit of claim 14, further comprising a second layer of metal disposed between the upper surface of the base plate and the first layer of metal.

16. The kit of claim 15, wherein the second layer of metal is made of a material selected from the group consisting of chromium and titanium.

17. The kit of claim 1, wherein the base plate is treated with a solution of mixed self-assembled monolayer (SAM).

18. The kit of claim 1, wherein the base plate is treated with a solution of biospecific mixed SAM.

19. The kit of claim 1, further comprising at least one registration element adapted to align with one another respective ones of the base plate, the first removable member and the second removable member.

20. The kit of claim 19, wherein the at least one registration element comprises a registration pin adapted to extend through the base plate, the first removable member, and the second removable member for aligning respective ones of the base plate, the first removable member and the second removable member.

21. The kit of claim 19, wherein the at least one registration element includes a visual mark disposed on at least one of the base plate, the first removable member and the second removable member.

22. The kit of claim 1, wherein at least one of the first removable member and the second removable member is made of PDMS.

23. The kit of claim 1 wherein the base plate is treated to enhance immobilization of peptide substrates thereon.

24. The kit of claim 1 wherein the base plate is treated to enhance immobilization of kinase substrates thereon.

25. The kit of claim 1, wherein the base plate is treated to enhance immobilization of antibody substrates thereon.

26. The kit of claim 25, wherein the base plate is treated with a solution of mixed self-assembled monolayer (SAM), such that binding of kinase substrates is enhanced in discrete areas and reduced or eliminated in others.

27. The kit of claim 26, wherein the base plate is treated with a solution of mixed SAM, such that specific binding of kinase substrates in desired orientations is enhanced and non-specific binding of kinase substrates is reduced or eliminated.

28. The kit of claim 27, wherein the base plate is treated with mixed SAM comprising about 0.1 to 20% maleimide-terminal groups in a background of tri(ethylene glycol) terminal groups.

29. A kit for forming device comprising:

a base plate; and

a removable member adapted to repeatedly self-seal on the base plate, the removable member defining at least one orifice therein and having walls bounding the at least one orifice on sides of the at least one orifice, the removable member further being configured such that, when it is placed in self-sealing contact with the base plate, it defines at least one well therewith corresponding to a respective one of the at least one orifice;

wherein the device is formed when the at least one well is defined by assembling the base plate and the removable member.

30. The kit of claim 29, wherein the walls of the removable member bounding the at least one orifice are tapered in a direction from an upper surface of the removable member toward a lower surface of the removable member such that an opening of the at least one orifice is larger in the upper surface of the removable member than an opening of the at least one orifice in the lower surface of the removable member.

31. The kit of claim 29, wherein the at least one orifice includes a plurality of orifices defining an array that spatially and dimensionally corresponds to a standard array of wells in an industry standard microtiter plate.

32. The kit of claim 31, wherein the standard microtiter plate is selected from the group consisting of a 6-well microtiter plate, a 12-well microtiter plate, a 24-well microtiter plate, a 96-well microtiter plate, a 384-well microtiter plate, a 1,536-well microtiter plate, and a 9,600-well microtiter plate.

33. The kit of claim 29, wherein the base plate is made of a material selected from the group consisting of glass, silicon, fused silica, metal films, polystyrene, poly(methylacrylate), and polycarbonate.

34. The kit of claim 29, further comprising a first layer of metal disposed on an upper surface of the base plate.

35. The kit of claim 34, wherein the first layer of metal is made of a material selected from a group consisting of gold, silver, platinum, palladium and copper.

36. The kit of claim 34, further comprising a second layer of metal disposed between the upper surface of the base plate and the first layer of metal.

37. The kit of claim 36, wherein the second layer of metal is made of a material selected from the group consisting of chromium and titanium.

38. The kit of claim 29, wherein the base plate is treated with a solution of mixed self-assembled monolayer (SAM).

39. The kit of claim 29, wherein the base plate is treated with a solution of biospecific mixed SAM.

40. The kit of claim 29, further comprising at least one registration element adapted to align with one another the base plate and the removable member.

41. The kit of claim 40, wherein the at least one registration element comprises a registration pin adapted to extend through the base plate and the removable member for aligning the base plate and the first removable member.

42. The kit of claim 40, wherein the at least one registration element includes a visual mark disposed on at least one of the base plate and the removable member.

43. The kit of claim 29, wherein the removable member is made of PDMS.

44. A kit for forming device comprising:

a base plate;

a first removable means for forming a plurality of first wells with the base plate, the first removable means being adapted to repeatedly self-seal on the base plate;

a second removable means for forming a plurality of second wells with at least one of the base plate an the first removable means, the second removable means being adapted to repeatedly self-seal on at least one of the first removable member and the base plate;

wherein the device is formed when at least one of the plurality of first wells and the plurality of second wells are defined by assembling selected ones of the base plate, the first removable means and the second removable means.