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Sensitive, multiplexed diagnostic assays for protein analysis

  • US 20030032049A1
  • Filed: 08/05/2002
  • Published: 02/13/2003
  • Est. Priority Date: 01/24/2000
  • Status: Active Grant
First Claim
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1. A method for detecting multiple different compounds in a sample, said method comprising:

  • (a) providing both a sample containing different compounds and a mixture of binding reagents, said binding reagents comprising nucleic acid-protein fusions, each fusion comprising (i) a protein portion which is known to specifically bind to one of said compounds and (ii) a nucleic acid portion which includes a unique identification tag;

    (b) contacting said sample with said binding reagents under conditions allowing said protein portions of said binding reagents and said compounds to form binding reagent-compound complexes;

    (c) capturing said binding reagent-compound complexes;

    (d) amplifying said unique identification tag included on said nucleic acid portions of said binding reagent-compound complexes; and

    (e) detecting said unique identification tag of each of said binding reagent-compound complexes, thereby detecting the corresponding compounds in said sample.

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