Biosensor

• US 20030032875A1
• Filed: 08/27/2002
• Published: 02/13/2003
• Est. Priority Date: 07/24/2000
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Inventors: Ikeda, Shin, Taniike, Yuko, Nankai, Shiro
• Granted Patent: US 6,885,196 B2
• Time in Patent Office: Days
• US Class Current: 600/368
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

The present invention provides a highly sensitive biosensor that needs a smaller amount of sample for measurement. The biosensor comprises a first insulating base plate having a working electrode, a second insulating base plate having a counter electrode opposed to the working electrode, a reagent layer comprising at least an oxidoreductase, and a sample supply pathway formed between the first and second insulating base plates, wherein the working electrode, counter electrode and reagent layer are exposed to an inside of the sample supply pathway, and the distance between the working electrode and the counter electrode is 150 μm or less.

Claims

1. A biosensor comprising a first insulating base plate having a working electrode, a second insulating base plate having a counter electrode opposed to said working electrode, a reagent layer comprising at least an oxidoreductase, and a sample supply pathway formed between the first and second insulating base plates, wherein said working electrode, counter electrode and reagent layer are exposed to an inside of said sample supply pathway, and the distance between said working electrode and said counter electrode is 150 μ

m or less.

2. The biosensor in accordance with claim 1, wherein the area of a portion of said counter electrode exposed to the sample supply pathway is equal to or smaller than the area of a portion of said working electrode exposed to the sample supply pathway.

3. The biosensor in accordance with claim 2, wherein the area of the portion of said working electrode exposed to the sample supply pathway is 0.1 to 2.0 mm², and the area of the portion of said counter electrode exposed to the sample supply pathway is 0.05 to 2.0 mm².

4. The biosensor in accordance with claim 1, wherein the distance between said working electrode and said counter electrode is 40 μ

m or more.

5. The biosensor in accordance with claim 1, wherein a spacer member having a slit is interposed between the first and second insulating base plates such that said sample supply pathway is formed in said slit.