Detection system

• US 20030036072A1
• Filed: 05/31/2002
• Published: 02/20/2003
• Est. Priority Date: 12/01/1999
• Status: Abandoned Application

• Current Assignee: DEFENCE, SECRETARY OF STATE FOR, THE
• Original Assignee: DEFENCE, SECRETARY OF STATE FOR, THE
• Inventors: Lee, Martin Alan, Leslie, Dario Lyall
• Application Number:
  US10/148,711
• Publication Number:
  US 20030036072A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/6844 Nucleic acid amplification reactions
• CPC Class Codes: C12Q 2561/113 Real time assay
• CPC Class Codes: C12Q 2563/113 the label being electroactive, e.g. redox labels
• CPC Class Codes: C12Q 2565/607 being a sensor, e.g. electrode
• CPC Class Codes: G01N 27/3277 being a redox reaction, e.g. detection by cyclic voltammetry voltammetry per se G01N27/42, G01N27/48

Abstract

A method for detecting a target nucleic acid sequence in a sample, by subjecting it to an amplification reaction and taking continuous electrochemical measurements on it during the reaction. The method can be used to determine whether an amplification reaction has taken place, to quantitate the amount of target in the sample or to determine sequence characteristics. Also disclosed is apparatus for use in the method, comprising (i) an amplification reaction vessel which comprises an electrochemical cell, (ii) means for taking continuous electrochemical measurements on a sample contained in the vessel and (iii) temperature control and measurement means, wherein the electrochemical cell comprises an element formed from an electrically conducting plastics material such as a polymer loaded with an electrically conducting material. Further disclosed is a reaction vessel for use in the apparatus, a probe for use in the method and a kit for effecting the method.

Claims

1. A method for detecting a target nucleic acid sequence in a sample, the method comprising subjecting the sample to an amplification reaction and taking continuous electrochemical measurements on the sample in situ during the amplification reaction, wherein the amplification reaction is conducted in the presence of an oligonucleotide probe that is specific for a region of the target nucleic acid sequence and includes an electrochemical label, which allows binding of the probe to the target sequence to be electrochemically monitored.

2. A method according to claim 1, wherein the electrochemical label comprises a modified base residue which has different electrochemical properties to native bases in the target sequence and otherwise present in the amplification reaction.

3. A method according to claim 2, wherein the modified base residue is inosine, xanthosine, hypoxanthine, xanthine, 1-methyladenine, 6-methyladenine, 6-benzyladenine, 8-oxyadenine or 2-aminopurine or a base which is modified by osmium tetroxide, pyridine or chloroacetaldehyde.

4. A method according to claim 1, 2 or 3, wherein the probe comprises a pair of labels which will undergo a detectable redox reaction when in close proximity to each other.

5. A method according to claim 4, wherein the probe comprises an oligonucleotide having complementary 5′

and 3′

sequences optionally contiguous with an amplification primer, a probe which is hydrolysed during the amplification reaction, a two-part probe the parts of which hybridise in close proximity to each other on the target sequence, or a probe having first and second labels separated by a region which forms a site for a restriction enzyme when in double stranded form.

6. A method according to any one of the preceding claims, wherein the probe is designed such that it can be released intact from the target sequence and so may take part again in the reaction.

7. A method according to any one of the preceding claims, wherein the probe is free in solution.

8. A method according to any one of the preceding claims, wherein the probe is specific either for a splice region of RNA or an intron in DNA, so that only one of amplified RNA or amplified DNA is detected.

9. A method according to any one of the preceding claims, wherein the amplification reaction is effected in the presence of a DNA binding agent which facilitates electrochemical measurement.

10. A method according to claim 9, wherein the DNA binding agent comprises an intercalating dye such as ethidium bromide, SybrGold, SybrGreen, PicoGreen, or acridine orange, a single stranded binding protein such as E. coli SSB, cisplatin or an electrochemical dye such as Hoechst 33258.

11. A method according to claim 9 or claim 10, wherein the electrochemical measurement is a result of interaction between the probe and the DNA binding agent.

12. A method for determining a characteristic of a target nucleic acid sequence, the method comprising carrying out a detection method according to any one of the preceding claims, and determining a particular reaction condition, characteristic of said sequence, at which the electrochemical measurements change as a result of destabilisation or formation of a duplex in the amplification reaction.

13. A method according to claim 12, wherein the characteristic condition is a polymorphism and/or allelic variation.

14. A method according to any one of the preceding claims, wherein the target sequence is an internal control sequence.

15. A method according to any one of the preceding claims, wherein the electrochemical measurements are temperature dependent electrochemical measurements.

16. A method according to any one of the preceding claims, wherein the electrochemical measurements are potentiometric, conductometric or amperometric, coulometric, and/or voltametric or polarographic measurements.

17. A method according to any one of the preceding claims, wherein the electrochemical measurements are used to determine whether and/or to what extent an amplification reaction has taken place.

18. A method according to any one of the preceding claims, wherein the electrochemical measurements are used to quantitate the amount of the target nucleic acid sequence in the sample.

19. A method according to any one of the preceding claims, wherein the electrochemical measurements are temperature dependent electrochemical measurements which allow characterisation of amplification species.

20. A method according to any one of the preceding claims, wherein the amplification reaction is a polymerase chain reaction (PCR), nucleic acid specific base amplification (NASBA), ligase chain reaction (LCR) or strand displacement amplification (SDA).

21. A method according to any one of the preceding claims, wherein the amplification reaction is a PCR.

23. A probe for use in a method according to any one of the preceding claims, which probe is free in solution.

40. An amplification reaction vessel for use as part of apparatus according to any one of claims 24 to 39 or in a method according to any one of claims 1 to 22, the vessel comprising an electrochemical cell which comprises at least one element formed from an electrically conducting plastics material.

41. An amplification reaction vessel according to claim 40, wherein the electrically conducting plastics material is a polymer loaded with an electrically conducting material.

42. An amplification reaction vessel according to claim 40 or claim 41, wherein the at least one plastics material element forms part of or is integral with the amplification reaction vessel.

43. A method according to any one of claims 1 to 22, involving the use of apparatus according to any one of claims 24 to 39, and/or an amplification reaction vessel according to any one of claims 40 to 42, to subject the sample to an amplification reaction and to take continuous electrochemical measurements of the sample during the amplification reaction.

44. A kit for use in a method according to any one of claims 1 to 22 or 43, the kit comprising at least one reagent required for the amplification reaction and a probe according to claim 23.

22. A method for detecting a target nucleic acid sequence in a sample, the method being substantially as herein described.

24. Apparatus for use in a method for detecting a target nucleic acid sequence in a sample, the apparatus comprising (i) an amplification reaction vessel which comprises an electrochenical cell, (ii) means for taking continuous electrochemical measurements on a sample contained in the amplification reaction vessel and (iii) temperature control and measurement means, wherein the electrochemical cell comprises at least one element formed from an electrically conducting plastics material.

25. Apparatus according to claim 24, wherein the means for taking electrochemical measurements allows the measurement of a temperature dependent electrochemical parameter.

26. Apparatus according to claim 24 or claim 25, wherein the means for taking electrochemical measurements allows potentiometric, conductometric or amperometric, coulometric, and/or voltametric or polarographic measurement.

27. Apparatus according to any one of claims 24 to 26, wherein the electrically conducting plastics material emits heat when an electric current is passed through it.

28. Apparatus according to claim 27, wherein the at least one plastics material element also functions as the temperature control means.

29. Apparatus according to any one of claims 24 to 28, wherein the electrically conducting plastics material is a polymer loaded with an electrically conducting material.

30. Apparatus according to claim 29, wherein the electrically conducting material is either carbon or a metal.

31. Apparatus according to any one of claims 24 to 30, wherein the at least one plastics material element forms part of or is integral with the amplification reaction vessel.

32. Apparatus according to claim 31, wherein the amplification reaction vessel is formed from the electrically conducting plastics material.

33. Apparatus according to any one of claims 24 to 32, wherein the electrochemical cell comprises a working electrode and a secondary electrode.

34. Apparatus according to claim 33, wherein the working electrode and/or the secondary electrode comprises an electrically conducting plastics material.

35. Apparatus according to any one of claims 24 to 34, wherein the electrochemical cell comprises a reference electrode.

36. Apparatus according to claim 35, wherein the reference electrode is a silver/silver chloride or a saturated calomel electrode.

37. Apparatus according to any one of claims 24 to 36, wherein the temperature control means allows thermal cycling of the contents of the amplification reaction vessel.

38. Apparatus according to any one of claims 24 to 37, which apparatus is microfabricated by lithographic etching of silicon wafer.

45. A kit according to claim 44, additionally comprising an amplification reaction vessel according to any one of claims 40 to 42, and/or apparatus according to any one of claims 24 to 39.

39. Apparatus for use in a method for detecting a target nucleic acid sequence in a sample, the apparatus being substantially as herein described.