Electron induced fluorescent method for nucleic acid sequencing
First Claim
1. A composition comprising a probe library, the library consisting of at least 256 different probes, wherein each different probe is distinguishably labeled.
1 Assignment
0 Petitions
Accused Products
Abstract
An apparatus, compositions and related methods for sequencing a target nucleic acid are described. In certain embodiments, the apparatus is a microfluidic apparatus comprising an input chamber, microchannel, output chamber and a detection unit that is operatively connected to the microchannel. In preferred embodiments, the methods include hybridizing a target nucleic acid to one or more probe libraries, moving the hybridized target nucleic acid past the detector, and detecting bound probes. Probe libraries may comprise oligonucleotides or oligonucleotide analogs, preferably with each probe uniquely labeled. A linear order of labeled probes hybridized to the target nucleic acid can be detected and the target nucleic acid sequence deduced. In preferred embodiments, probe labels are detected by analysis of electron-induced fluorescence of probes labeled with conductive polymers.
-
Citations
26 Claims
- 1. A composition comprising a probe library, the library consisting of at least 256 different probes, wherein each different probe is distinguishably labeled.
-
9. A method of sequencing a target nucleic acid comprising:
-
a) obtaining a target nucleic acid;
b) hybridizing the nucleic acid to a probe library, the library consisting of at least 256 different probes, wherein each different probe is distinguishably labeled;
c) separating the hybridized nucleic acid from unhybridized probes; and
d) detecting the order of hybridized probes along the nucleic acid. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17)
-
-
18. A nucleic acid sequencing apparatus comprising:
-
a) an input chamber;
b) a microchannel in fluid communication with the input chamber; and
c) a detection unit operably coupled to the microchannel, the detection unit comprising an electron beam source and a detector. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26)
-
Specification