Coplanar waveguide biosensor for detecting molecular or cellular events

• US 20030040004A1
• Filed: 08/23/2002
• Published: 02/27/2003
• Est. Priority Date: 02/02/1998
• Status: Active Grant

• Current Assignee: DH Technologies Development Pte. Ltd. (Danaher Corporation)
• Original Assignee: Signature BioScience, Inc.
• Inventors: Rhodes, Mark A., Kramer, Kurt, Bartell, Barrett, Hefti, John J.
• Granted Patent: US 7,083,985 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 2223/419 computed tomograph
• CPC Class Codes: G01N 23/046 using tomography, e.g. computed tomography [CT]
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property

Abstract

A coplanar waveguide biosensor and methods of use include a coplanar waveguide transmission line and a sample containment structure. The coplanar waveguide transmission line is operable to support the propagation of an electromagnetic signal and includes a signal line and one or more spaced apart ground elements. The signal line is configured to conduct a time-varying voltage, and the one or more ground elements are configured to maintain a time-invariant voltage, a detection region being formed between a portion of the signal line and a portion of at least one of the one or more ground elements. Detection methods are improved through the enhancement of the electric field in the detection region via impedance discontinuities in the signal line and ground elements. The sample containment structure intersects the detection region of the coplanar waveguide transmission line and includes a cavity configured to hold 1 ml or less of sample solution within the detection region.

Claims

1. A coplanar waveguide biosensor for detecting molecular or cellular events, comprising:

a one-port coplanar waveguide transmission line operable to support the propagation of a electromagnetic test signal, comprising;

a signal line configured to conduct a time-varying voltage therealong; and

one or more ground elements configured to maintain a time-invariant voltage therealong, the one or more ground elements spaced apart from the signal line and located generally within the same plane as the signal line, wherein a detection region is formed between a portion of the signal line and a portion of at least one of the one or more ground elements; and

a sample containment structure intersecting the detection region of the one-port coplanar waveguide transmission line, wherein the sample containment structure comprises a cavity operable to hold 1 ml or less of sample solution within the detection region.

2. The coplanar waveguide biosensor of claim 1, wherein the signal line comprises a tapered section.

3. The coplanar waveguide biosensor of claim 1, wherein the one or more ground elements comprises a tapered section.

4. The coplanar waveguide biosensor of claim 1,wherein the signal line comprises a tapered section and the one or more ground elements comprises a tapered section.

5. The coplanar waveguide biosensor of claim 1, wherein the coplanar waveguide transmission line comprises a resonant structure.

7. The method of claim 1 wherein the coplanar waveguide transmission line is a one-port coplanar waveguide transmission line.

8. The method of claim 1 wherein the coplanar waveguide transmission line is a two-port coplanar waveguide transmission line.

9. The method of claim 1, wherein the sample containment structure comprises a well structure.

10. The method of claim 1, wherein the sample containment structure comprises a fluid channel.

11. The method of claim 1, wherein the sample containment structure

comprises a flow tube.

12. The method of claim 1, wherein the signal line comprises a tapered section.

13. The method of claim 1,wherein the one or more ground elements comprises a tapered section.

14. The method of claim 1,wherein the signal line comprises a tapered section and the one or more ground elements comprises a tapered section.

15. The method of claim 1, wherein the coplanar waveguide transmission line comprises a resonant structure.

16. The method of claim 1, further comprising providing at least one sample port operable to supply the sample solution to the cavity.

17. The method of claim 1, wherein the molecular event is directly or indirectly physically attached to the coplanar waveguide transmission line within the detection region

18. The method of claim 1, wherein the molecular event is separated from the coplanar waveguide transmission line within the detection region.

19. The method of claim 1 , wherein the cellular event is directly or indirectly physically attached to the coplanar waveguide transmission line within the detection region

20. The method of claim 1, wherein the cellular event is separated from the coplanar waveguide transmission line within the detection region.

22. The method of claim 16, wherein the signal detector comprises a network analyzer.

23. The method of claim 16, wherein the signal detector comprises a vector voltmeter.

24. The method of claim 16, wherein the signal source is operable to output one or more signals from 300 KHz to 3 GHz.

25. The method of claim 16, wherein the signal source is operable to output one or more signals from 45 MHz to 26 GHz.

6. A method for detecting a molecular or cellular event using a coplanar waveguide, comprising:

providing a coplanar waveguide transmission line operable to support the propagation of a electromagnetic test signal,

the coplanar waveguide comprising;

a signal line configured to conduct a time-varying voltage there along; and

one or more ground elements configured to maintain a time-invariant voltage there along, the one or more ground elements spaced apart from the signal line and located generally within the same plane as the signal line, wherein a detection region is formed between a portion of the signal line and a portion of at least one of the one or more ground elements;

providing a sample containment structure intersecting the detection region of the coplanar waveguide transmission line, wherein the sample containment structure comprises a cavity operable to hold 1 ml or less of sample solution within the detection region,

propagating an incident test signal along the coplanar waveguide signal line to the detection region,

electromagnetically coupling at least a portion of the incident test signal to the molecular or cellular event, and

measuring the modulation of the incident test signal wherein the signal modulation is indicative of the presence and identity of the molecular event.

21. A method for detecting a molecular or cellular event using a coplanar waveguide, comprising:

providing a signal source operable to output an electromagnetic test signal;

providing a coplanar waveguide transmission line electrically coupled to the signal source and operable to support the propagation of a electromagnetic signal,

the coplanar waveguide transmission line comprising;

a signal line configured to conduct a time-varying voltage there along; and

one or more ground elements configured to maintain a time-invariant voltage there along, the one or more ground elements spaced apart from the signal line and located generally within the same plane as the signal line, wherein a detection region is formed between a portion of the signal line and a portion of at least one of the one or more ground elements;

providing a sample containment structure intersecting the detection region of the one-port coplanar waveguide transmission line, wherein the sample containment structure comprises a cavity operable to hold 1 ml or less of sample solution within the detection region,

providing a signal detector electrically coupled to the coplanar waveguide signal line,

propagating an incident test signal along the coplanar waveguide signal line to the detection region,

electromagnetically coupling at least a portion of the incident test signal to the molecular or cellular event, and

measuring the modulation of the incident test signal wherein the signal modulation is indicative of the presence and identity of the molecular event.

26. A coplanar waveguide biosensor for detecting molecular or cellular events, comprising:

a coplanar waveguide transmission line operable to support the propagation of a electromagnetic test signal, comprising;

a signal line configured to conduct a time-varying voltage there along, the signal line comprising a tapered section; and

one or more ground elements configured to maintain a time-invariant voltage therealong, the one or more ground elements spaced apart from the signal line and located generally within the same plane as the signal line, wherein a detection region is formed between a portion of the signal line and a portion of at least one of the one or more ground elements; and

a sample containment structure intersecting the detection region of the one-port coplanar waveguide transmission line, wherein the sample containment structure comprises a cavity operable to hold 1 ml or less of sample solution within the detection region.

27. A coplanar waveguide biosensor for detecting molecular or cellular events, comprising:

a coplanar waveguide transmission line operable to support the propagation of a electromagnetic test signal, comprising;

a signal line configured to conduct a time-varying voltage there along; and

one or more ground elements configured to maintain a time-invariant voltage therealong, the one or more ground elements spaced apart from the signal line and located generally within the same plane as the signal line, wherein a detection region is formed between a portion of the signal line and a portion of at least one of the one or more ground elements, the one or more ground elements comprising a tapered section; and

a sample containment structure intersecting the detection region of the one-port coplanar waveguide transmission line, wherein the sample containment structure comprises a cavity operable to hold 1 ml or less of sample solution within the detection region.

28. A coplanar waveguide biosensor for detecting molecular or cellular events, comprising:

a coplanar waveguide transmission line operable to support the propagation of a electromagnetic test signal, comprising;

a signal line configured to conduct a time-varying voltage there along, the signal line comprising a tapered section; and

one or more ground elements configured to maintain a time-invariant voltage therealong, the one or more ground elements spaced apart from the signal line and located generally within the same plane as the signal line, wherein a detection region is formed between a portion of the signal line and a portion of at least one of the one or more ground elements, the one or more ground elements comprising a tapered section; and

a sample containment structure intersecting the detection region of the one-port coplanar waveguide transmission line, wherein the sample containment structure comprises a cavity operable to hold 1 ml or less of sample solution within the detection region.

29. A coplanar waveguide biosensor for detecting molecular or cellular events, comprising:

a coplanar waveguide transmission line comprising a resonant structure and operable to support the propagation of a electromagnetic test signal, comprising;

a signal line configured to conduct a time-varying voltage there along; and

one or more ground elements configured to maintain a time-invariant voltage therealong, the one or more ground elements spaced apart from the signal line and located generally within the same plane as the signal line, wherein a detection region is formed between a portion of the signal line and a portion of at least one of the one or more ground elements; and

a sample containment structure intersecting the detection region of the one-port coplanar waveguide transmission line, wherein the sample containment structure comprises a cavity operable to hold 1 ml or less of sample solution within the detection region.

30. A method for detecting a molecular or celluar event using a coplanar waveguide, comprising:

providing a coplanar waveguide transmission line operable to support the propagation of an electromagnetic test signal,

the coplanar wave guide comprising a resonant structure,

a signal line configured to conduct a time-varying voltage there along, and one or more ground elements configured to maintain a time-invariant voltage therealong, the one or more ground elements spaced apart from the signal line and located generally within the same plane as the signal line, wherein a detection region is formed between a portion of the signal line and a portion of at least one of the one or more ground elements;

providing a sample containment structure intersecting the detection region of the one-port coplanar waveguide transmission line, wherein the sample containment structure comprises a cavity operable to hold 1 ml or less of sample solution within the detection region.

propagating an incident test signal along the coplanar waveguide signal line to the detection region,

electromagnetically coupling at least a portion of the incident test signal to the molecular or cellular event, and

measuring the modulation of the incident test signal wherein the signal modulation is indicative of the presence and identity of the molecular event.