Binding assays using magnetically immobilized arrays
First Claim
1. A magnetic particle array for performing biological and chemical assays, the magnetic particle array comprising:
- at least one magnetic layer comprising a predetermined spatial distribution of spaced-apart magnetized spots;
a plurality of magnetic particles immobilized to said magnetic spots;
wherein at least a plurality of said particles comprise different molecules.
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Abstract
Systems and methods for preparing and using magnetic particle arrays are provided. In conventional assays, a target molecule is immobilized in a particular array position on the surface of the substrate by chemically conjugating the molecule to the surface of the substrate. According to embodiments of the present invention, target biomolecules are immobilized magnetically rather than chemically. Accordingly, the target molecules are chemically conjugated to the surface of a magnetic particle, and it is the magnetic particles that are positioned in an array by printing (or spotting) the magnetic particles onto the surface of a magnetic array substrate. The array is exposed to a solution of probe molecules (analytes) having detector labels, and the positions in the array where complementary target binds to probe are recorded. Such magnetic particle arrays may be used in a variety of applications, including drug screening, nucleic acid sequencing, mutation analysis, medical diagnosis, and immunoassay analysis. The magnetic particle array may be The advantages of a magnetic particle array, which may be configured with a holder having microfluidic channels to deliver sample to the array to comprise a magnetic array biochip, include reduced assay variability, enhanced flexibility, lower cost and higher throughput.
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Citations
41 Claims
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1. A magnetic particle array for performing biological and chemical assays, the magnetic particle array comprising:
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at least one magnetic layer comprising a predetermined spatial distribution of spaced-apart magnetized spots;
a plurality of magnetic particles immobilized to said magnetic spots;
wherein at least a plurality of said particles comprise different molecules. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
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32. A method of making the said magnetic particle array comprising;
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to a magnetic array layer comprised of plurality of magnetized spots, dispensing or microspotting reagent containing magnetic particles on the surface of the magnetic layer in spatially defined and physically addressable manner until the said magnetic array is formed.
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33. A magnetic particle array comprising;
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a magnet layer having individual permanently magnetized spots at a density in the range of about 1 to 100 spots per mm2, each of said spots having a maximum planar dimension in the range of about 1 um to 5 mm;
immobilized at a plurality of said spots magnetic particles having diameter in the range of about 0.005 to 1,000 um, a plurality of said magnetic particles having different molecules of interest conjugated to said magnetic particles.
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34. A method of using a magnetic particle array to perform an assay employing magnetic particles to which target molecules have been conjugated and a magnetic array substrate having spaced-apart magnetized spots, the method comprising:
transferring the said magnetic particles onto the surface of the magnetic array substrate with said magnetic particles being immobilized at said spaced apart magnetized spots to form a magnetic particle array with the said magnetic particles in a predetermined organization; and
adding a solution comprising a composition of interest to said magnetic particle array; and
determining any interaction between said composition of interest and said target molecule.- View Dependent Claims (35, 36, 37)
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38. A system for screening reaction between compositions of interest, said system comprising;
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vessels organized in a spatial arrangement containing magnetic particles, wherein a plurality of said vessels contain magnetic particles having different composition of interest;
a magnetic layer comprising magnetized spots spatially organized in conformance with said vessel spatial arrangement; and
a magnetic transfer device for simultaneously retrieving magnetic particles from each of the said vessels and transferring said magnetic particles to said magnetic layer in the spatial organization of said vessels. - View Dependent Claims (39, 40, 41)
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Specification