Preparation and xenotransplantation of porcine islets
First Claim
1. A method of preparing a xenotransplantable porcine islet preparation capable upon xenotransplantation of producing porcine insulin in an appropriate recipient mammal, the method including or comprising the steps of:
- (I) harvesting the pancreas of piglets at or near full term gestation, and (ii) extracting pancreatic β
islet cells from the harvested pancreas wherein the islets (at least at some stage in the performance of the method) are exposed to nicotinamide.
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Accused Products
Abstract
The invention relates to developments in the treatment of diabetes in mammals. Particularly it relates to a method of preparing a xenotransplantable porcine islet preparation capable upon xenotransplantation of producing porcine insulin in an appropriate recipient mammal, the method including or comprising the steps of:
(I) harvesting the pancreas of piglets at or near full term gestation, and
(ii) extracting pancreatic β islet cells from the harvested pancreas wherein the islets (at least at some stage in the performance of the method) are exposed to nicotinamide.
Further, the invention relates to a method of encapsulation of a xenotransplantable porcine islet preparation, and transplantation of such a preparation, or a capsule containing such a preparation, into an appropriate recipient mammal.
42 Citations
109 Claims
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1. A method of preparing a xenotransplantable porcine islet preparation capable upon xenotransplantation of producing porcine insulin in an appropriate recipient mammal, the method including or comprising the steps of:
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(I) harvesting the pancreas of piglets at or near full term gestation, and (ii) extracting pancreatic β
islet cells from the harvested pancreaswherein the islets (at least at some stage in the performance of the method) are exposed to nicotinamide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 54, 55, 107)
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19. A method of preparing a xenotransplantable porcine islet preparation capable upon xenotransplantation of producing porcine insulin in an appropriate recipient mammal, said method including or comprising the steps of:
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(I) harvesting the pancreas of piglets at or near full term gestation, and (ii) preparing a culture of the pancreatic β
islet cells(iii) simultaneously with step (ii) and/or after step (ii) extracting pancreatic β
islet cells from the culture of the harvested pancreas and(iv) encapsulating the islet cells with a biocompatible xenotransplantable material, said material in vivo being both glucose and insulin porous, wherein nicotinamide is introduced to the islets or islet cells prior to encapsulation at any one or more stages of the procedure. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93)
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56. A xenotransplantable capsule of at least one porcine pancreatic β
- islet cell comprising at least one viable porcine pancreatic β
islet cell enclosed in an in vivo glucose porous and insulin porous biocompatible material. - View Dependent Claims (106)
- islet cell comprising at least one viable porcine pancreatic β
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57. A method for treatment of a mammalian patient suffering from diabetes which comprises:
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(a) extracting pancreatic β
islet cells from piglets at or near full term gestation;
(b) Simultaneously with, and/or after (a), treating said islets with nicotinamide, (c) encapsulating said islets in a biocompatible material which will allow in vivo glucose movement to and insulin movement from the islets, and (d) injecting or otherwise implanting the encapsulated islet cells of step (c) so as to transplant into said mammalian patient an effective amount of viable piglet islet cells capable of producing insulin in the patient, - View Dependent Claims (58, 59)
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94. A method for the treatment of a mammalian patient suffering from or predisposed to diabetes, said method including or comprising the steps of:
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(A) (i) harvesting the pancreas of piglets at or near full term gestation, (ii) culturing the harvested pancreas in Mammalian Albumin substantially free of non-human microbiological agents, (iii) simultaneously with step (ii) and/or after step (ii), extracting the islets from the harvested pancreas using a suitable Liberase®
,wherein the islets (at least at some stage in the performance of (A)) are exposed to nicotinamide;
(B) (i) encapsulating the islets prepared by (A) with a suitable encapsulation material that allows both glucose and insulin movement therethrough, and (ii) implanting the encapsulated porcine islets into the recipient mammal. - View Dependent Claims (95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105)
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108. A method of preparing a xenotransplantable porcine islet preparation capable upon xenotransplantation of producing porcine insulin in an appropriate recipient mammal substantially as herein described with reference to one or more of the Figures and/or Examples.
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109. A method for treatment of a mammalian patient suffering from diabetes substantially as herein described with reference to one or more of the Figures and/or Examples.
Specification