Biomolecule array

• US 20030044833A1
• Filed: 09/04/2002
• Published: 03/06/2003
• Est. Priority Date: 09/06/2001
• Status: Abandoned Application

• Current Assignee: Randox Laboratories Limited, The Torrington Company (The Timken Company)
• Original Assignee: Randox Laboratories Limited
• Inventors: Benchikh, Elouard, McConnell, Robert Ivan, Fitzgerald, Stephen Peter, Lamont, John Victor
• Application Number:
  US10/233,419
• Publication Number:
  US 20030044833A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: G01N 33/551 the carrier being inorganic

Abstract

Multi-analyte microarrays are produced by first attaching to the surface of a solid support, a silane with an electrophilic terminal group, contacting the terminal group with a polyamine, and activating the polyamine with an electrophilic, bifunctional linker.

Claims

1. A process for the manufacture of a solid support comprising a linker molecule capable of attaching a biomolecule to the solid support, comprising:

attaching to the surface of a solid support, a silane with an electrophilic terminal group;

contacting the terminal group with a polyamine, and

activating the polyamine with an electrophilic, bifunctional linker.

2. A process according to claim 1, wherein the silane is an alkoxy silane.

3. A process according to claim 1 or claim 2, wherein the silane is GOPTS or GOPS MDM

4. A process according to any preceding claim, wherein the polyamine is an amine-terminated non-linear or dendrimeric molecule.

5. A process according to any of claims 1 to 3, wherein the polyamine is amino-modified dextran.

6. A process according to any preceding claim, wherein the electrophilic, bifunctional linker has the general formula:

X—

R—

X

wherein R is an alkyl, aryl or heteroaryl group, and X is selected from the group consisting of isothiocyanate, isocyanate, epoxide, carbonylimidazole and N-hydroxysuccinimide.

7. A process according to any preceding claim, wherein the electrophilic, bifunctional linker is PDITC

8. A process according to any preceding claim, wherein the solid support is a ceramic material.

9. A process according to any preceding claim, further comprising the attachment of an amino-capped biomolecule.

10. A process according to claim 9, wherein the biomolecule is a polynucleotide or a protein.

11. A functionalised solid support material obtainable by a process according to any preceding claim.

12. Use of a solid support material according to claim 11, in a diagnostic assay procedure.

13. Use according to claim 12, wherein the assay is a DNA hybridisation assay