Microarrays and their manufacture by slicing
First Claim
Patent Images
1. A fiber bundle comprising a plurality of fibers attached to each other in a fixed position with respect to each other wherein the fibers have different agents of interest immobilized in or on different fibers.
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Abstract
Microarrays are prepared by using a separate fiber for each compound being used in the microarray. The fibers are bundled and sectioned to form a thin microarray that may be glued to a backing.
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Citations
80 Claims
- 1. A fiber bundle comprising a plurality of fibers attached to each other in a fixed position with respect to each other wherein the fibers have different agents of interest immobilized in or on different fibers.
- 19. An array comprising a plurality of cells in a known location on the array, each cell containing an agent of interest immobilized in or on at least a portion of a fiber, wherein different cells contain different fibers or portions of a fiber which contains a different agent of interest immobilized therein or thereon, and wherein each agent of interest is located in a known cell.
- 30. A microarray comprising a solid phase support and at least about 500 cells per square centimeter wherein each cell contains a agent of interest that is not chemically bound to the solid phase support.
- 32. A microarray comprising a solid phase support and at least about 500 cells per square centimeter wherein each cell contains an agent of interest which is a macromolecule, a microorganism, a plant or animal cell, an organelle or a fraction of a biological cell.
- 34. A microarray comprising a solid phase support and at least about 500 cells per square centimeter wherein each cell contains an agent of interest that was synthesized prior to contacting the solid phase support.
- 36. A microarray comprising a solid phase support and at least about 500 different cells per square centimeter wherein each cell is formed by a solid material adhered to said solid phase support wherein each solid material contains a agent of interest.
- 38. A multiwell plate containing at least about 500 wells per square centimeter.
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40. A thin elongated fiber impregnated with a solid phase wherein the solid phase is bound to a agent of interest.
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41. A solid phase construct containing an immobilized agent of interest comprising an embedding medium, a porous or hollow solid phase and a agent of interest,
wherein the agent of interest is immobilized on inside surfaces of the porous or hollow solid phase, wherein the porous or hollow solid phase is embedded in the embedding medium, and wherein the inside surfaces are exposed to the surface of the construct by cleaving such that individual porous or hollow solid phases are cleaved in plural sections.
- 42. A microarray containing a plurality of different cells wherein each cell contains a solid phase support, a porous particle containing a agent of interest immobilized on an inside surface of a porous particle and a medium for attaching said particle to said solid phase support in a particular cell.
- 44. An elongated fiber having an agent of interest immobilized thereon or therein such that a detectable number of single agents of interest are present in each millimeter of fiber length.
- 51. A fiber cross-section structure comprising a cross-section of at least two fibers, each fiber being set in parallel juxtaposition to another fiber, and at least two agents of interest being immobilized in or on the fibers, wherein each fiber contains a different agent of interest.
- 56. A microarray comprising a solid block on an inert solid support, the solid block containing a plurality of cells exposed to the surface of the block wherein each cell contains a different agent of interest and was independently prepared and embedded into the solid block before mounting on the inert solid support.
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66. A microarray comprising:
- a solid phase having a surface, and a plurality of structures bound to said surface, wherein each of a plurality of structures has an immobilized agent of interest available for binding to a target, wherein the target is a binding partner for the agent of interest, and wherein the microarray contains a plurality of different agents of interest in a corresponding plurality of different structures.
- View Dependent Claims (67, 68, 69, 70)
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71. A microarray comprising:
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a solid phase having a flat surface, and a plurality of structures projecting away from the plane of the solid phase, wherein each of a plurality of the structures an agent of interest, wherein the agent of interest is available for binding to a target in a sample applied to the microarray, wherein the target is a binding partner for the agent of interest, and wherein the microarray contains a plurality of different agents of interest in a corresponding plurality of different structures. - View Dependent Claims (72, 73)
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74. A method of manufacturing a microarray device comprising:
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synthesizing at least one functional moiety onto a plurality of fibers wherein one or more fibers receives at least one moiety, bundling said plurality of fibers in a predetermined arrangement;
bonding or fixing said bundled plurality of fibers to fix the positions of the fibers;
and cleaving said bundled fibers into a plurality of chips to be deposited at a specific address on a solid phase. - View Dependent Claims (75, 76, 77, 78, 79, 80)
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Specification