Bio-reaction process and product
First Claim
1. A process for producing product enriched for one or more enzymes selected from the group cellulase, amylase, protease, or lipase (a) providing a first-stage bio-reaction feedstock in a solid substrate bio-reaction mixture containing microbial growth nutrients, in a bio-reactor (b) inoculating the first-stage feedstock with a culture of a microorganism capable of secreting an enzyme selected from the group cellulase, amylase, protease, or lipase thereby producing a first bio-reaction mixture (c) incubating the first bio-reaction mixture for a time sufficient to enrich the bio-reaction mixture with said enzyme.
1 Assignment
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Accused Products
Abstract
Rural biomass and other cellulosic materials are converted to a protein-enriched animal feed supplement or to single-cell protein by a series of bio-reactions. A first stage bio-reaction is a solid substrate bio-reaction. Enzymes, such as cellulase, produced by the first-stage bio-reaction are added to a second-stage bio-reaction. Raw second-stage bio-reaction feedstock is pretreated to hydrolyze hemicellulose and/or to partially digest starch in the feedstock. In the second-stage bio-reaction, the feedstock is substantially digested and single-cell protein is harvested in an aerobic bio-reaction, while ethanol is produced in an anaerobic reaction. Alternatively, raw biomass or other cellulosic materials can be treated with organic acid (e.g. maleic acid) combined with dry steam to produce a nutritional product that can be directly used as an animal feed supplement.
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Citations
41 Claims
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1. A process for producing product enriched for one or more enzymes selected from the group cellulase, amylase, protease, or lipase
(a) providing a first-stage bio-reaction feedstock in a solid substrate bio-reaction mixture containing microbial growth nutrients, in a bio-reactor (b) inoculating the first-stage feedstock with a culture of a microorganism capable of secreting an enzyme selected from the group cellulase, amylase, protease, or lipase thereby producing a first bio-reaction mixture (c) incubating the first bio-reaction mixture for a time sufficient to enrich the bio-reaction mixture with said enzyme.
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34. A process for producing a nutritional product comprising the steps of:
- (a) treating a raw feedstock or biomass with acid combined with steam for a time sufficient to hydrolyze hemicellulose and cellulose in the raw feedstock or biomass, and (b) neutralizing the acid in the reaction mix of step (a), whereby a nutritional product is prepared.
- View Dependent Claims (35, 36, 37, 38, 39, 40, 41)
Specification