Accurate instrumetation for optical measurement of samples

• US 20030048447A1
• Filed: 09/07/2001
• Published: 03/13/2003
• Est. Priority Date: 09/07/2001
• Status: Active Grant

• Current Assignee: Wallac Oy (Revvity, Inc.)
• Original Assignee: Wallac Oy (Revvity, Inc.)
• Inventors: Harju, Raimo, Isaksson, Christer
• Granted Patent: US 6,891,618 B2
• Time in Patent Office: Days
• US Class Current: 356/417
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/0275 making use of sensor-related data, e.g. for identification of sensor parts or optical elements
• CPC Class Codes: G01N 2021/6471 Special filters, filter wheel
• CPC Class Codes: G01N 2021/6482 Sample cells, cuvettes
• CPC Class Codes: G01N 2021/6484 Optical fibres
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence

Abstract

The present invention relates generally to the field of biochemical laboratory. More particularly the invention relates to the improved and more accurate instrumental features of equipment used as e.g. fluorometers, photometers and luminometers. The object of the invention is achieved by providing an optical measurement instrument where there is an interface (218, 223, 233a, 233b, 238) for a changeable optical module (240), and excitation and/or emission beam is guided through an aperture of the optical module. This allows performing various types of measurements by changing an optical module without a problem lenses becoming unclean due to manual handling of the optical modules. The invention also makes possible to achieve an accurate measurement location and area within a sample.

Claims

1. An optical measurement instrument for measuring samples, comprising an illumination source (211) for forming an excitation beam, a detector (231a) for detecting an emission beam, an interface (218, 223, 233a, 233b, 238) for a changeable optical module (240) with a purpose of directing the excitation beam received from the illumination source (211) into the sample (281) and/or directing an emission beam received from the sample (281) to a detector (231 a), characterized in that the interface further comprises means (238, 233b) for adapting to at least one of said beams being transmitted through an aperture located on a wall of an optical module.

2. An instrument according to claim 1, characterized in that said interface comprises means for adapting to a beam, which is substantially focused in said aperture.

3. An instrument according to claim 1, characterized in that the size of the aperture is arranged to determine the size of the sample area to be measured.

4. An instrument according to claim 1, characterized in that it comprises a first mirror (241) for reflecting the excitation beam received from an illumination source into the sample and for transmitting an emission beam received from the sample.

5. An instrument according to claim 1, characterized in that the instrument comprises a second detector, and the optical module comprises means (242) for dividing the emission beam into a first emission beam for the first detector a second emission beam for the second detector.

6. An instrument according to claim 5, characterized in that said means for dividing the emission beam received from the sample into two emission beams is a second dichroic mirror (242), said mirror transmitting the first emission beam and reflecting the second emission beam.

7. An instrument according to claim 1, characterized in that said interface further comprises means (219) for receiving an illumination reference beam from a further aperture of the optical module.

8. An instrument according to claim 7, characterized in that the changeable optical module comprises a third mirror (243), which is a beam splitter mirror for reflecting a part of the light received from the illumination source (211), and the instrument comprises a further detector (219) for measuring the reflected, reference part of the excitation light in order to measure the illuminating effect of the illumination source.

9. An instrument according to claim 8, characterized in that the transmission/reflection properties of said third mirror are application specific.

10. An instrument according to claim 1, characterized in that it comprises a top measurement head (320) for measuring a sample from (320) above the sample (381), wherein the top measurement head comprises a top optical module (340).

11. An instrument according to claim 10, characterized in that the top measurement head (320) comprises means (318T, 340a, 323) for providing an excitation beam to the sample and means (323, 340a, 337a) for measuring a first emission beam from the sample.

12. An instrument according to claim 11, characterized in that the top measurement head comprises means (323, 340a, 338, 337b) for measuring a second emission beam from the sample.

13. An instrument according to claim 1, characterized in that it comprises a bottom measurement head (360) for measuring a sample from below the sample (381), wherein said bottom measurement head (360) comprises a changeable bottom optical module (350) and an optical interface for the bottom optical module.

14. An instrument according to claim 13, characterized in that it comprises an optical fibre (318B) for guiding light between the illumination source and the bottom optical module (350).

15. An instrument according to claim 13, characterized in that it comprises an optical fibre (338a) for guiding the first emission beam between the bottom optical module (350) and the first detector (332a).

16. An instrument according to claim 14, characterized in that the optical interface for the bottom optical module comprises means (338a) for receiving a second emission beam from the optical module (350).

18. An instrument according to claim 14, characterized in that it comprises an optical fibre (338b) for directing the second emission beam between the bottom optical module (350) and the second detector (332b).

19. An instrument according to claim 1, characterized in that said aperture is optically adapted to an end of an optical fibre.

20. A changeable optical module (1340, 1850) for an optical measurement instrument, the module comprising

means (1346, 1856) for receiving an excitation beam from an illumination source and means (1341, 1348, 1851, 1858) for directing the excitation beam to a sample, and/or

means (1348) for receiving an emission beam from the sample and means (1341, 1344, 1851, 1854) for outputting the emission beam received from the sample to a detector,

characterized in that the means for receiving and/or the means for outputting comprise(s) an aperture on an outer wall of the optical module module.

21. A changeable optical module according to claim 20, characterized in that the aperture is adapted to a beam, which is substantially focused in said aperture.

22. A changeable optical module according to claim 20, characterized in that the size of the aperture is arranged to determine the size of the sample area to be measured.

23. A changeable optical module according to claim 20, characterized in that it comprises a first mirror (1341) for reflecting the excitation beam received from an illumination source into the sample and for transmitting an emission beam received from the sample.

24. A changeable optical module according to claim 20, characterized in that it comprises a second mirror, which is a dichroic mirror for separating the emission beam received from the sample into two emission beams (1342), said mirror transmitting the first emission beam and reflecting the second emission beam.

25. A changeable optical module according to claim 24, characterized in that said second mirror (1342) is substantially transparent to light of a first wavelength band for transmitting the first emission within said first wavelength band into the first detector, and said second mirror is substantially reflective to light of a second wavelength band for reflecting the second emission within said second wavelength band into the second detector.

26. A changeable optical module according to claim 20, characterized in that the module comprises a third mirror (1343) for reflecting a part of the excitation beam to a detector for measuring the illuminating effect, wherein said third mirror is a beam splitter mirror.

27. A changeable optical module according to claim 26, characterized in that the transmission/reflection properties of said third mirror (1343) are application specific.

28. A changeable optical module according to claim 26, characterized in that it comprises an aperture for outputting said reflected part of the excitation beam.

29. A changeable optical module according to claim 20, characterized in that the means for outputting the excitation beam is the same as the means for receiving the emission beam (1348).

30. A changeable optical module according to claim 29, characterized in that said means is an aperture.

31. A changeable optical module according to claim 20, characterized in that said means for outputting the emission beam is an aperture (1344).

32. A changeable optical module according to claim 20, characterized in that it comprises an aperture (1345) for outputting a second emission beam.

33. A changeable optical module according to claim 20, characterized in that said module is adapted for measurement from above a sample to be measured (1340).

34. A changeable optical module according to claim 20, characterized in that said module is adapted for measurement from below a sample to be measured (1850).

35. A changeable optical module according to claim 20, characterized in that said module is adapted to output the first emission beam to an end of an optical fibre (1854).

36. A changeable optical module according to claim 20, characterized in that said module is adapted to output said second emission beam to an end of an optical fibre (1855).

37. A changeable optical module according to claim 20, characterized in that said means for receiving excitation beam is an aperture (1346).

38. A changeable optical module according to claim 37, characterized in that said aperture is adapted for receiving the excitation beam from the end of an optical fibre.

39. A changeable optical module according to claim 20, characterized in that at least one aperture is located on a wall, said wall or part of it being changeable for changing the size of the aperture.

40. A changeable optical module according to claim 20, characterized in that said module comprises attachment means for manual attachment and detachment of the module to/from the optical measurement instrument.

41. A changeable optical module according to claim 20, characterized in that said module comprises means for attaching to movable base (1228) of an optical measurement instrument, wherein the change of a module used for a measurement is arranged by moving said movable base.

42. A process for measurement of samples with an optical measurement instrument comprising at least one measurement head, possible means for providing excitation of a sample and means for measuring an emission from the sample, the process comprising the phases of

selecting a measurement mode (11),

selecting at least one changeable optical module for a measurement head, the optical module guiding the excitation beam from an illumination source into the sample (20, 21) and/or guiding at least one emission beam from a sample into at least one detector (20, 21),

performing the selected optical measurement (23-25),

characterized in that in the step of selecting the optical module, a kind of optical module is selected, which has an aperture for receiving an excitation beam and/or for outputting an emission beam.

43. A method for optical measurement of samples comprising the step of transmitting excitation/emission beam between the sample and a measurement head of an optical instrument, comprising transmitting said beam through an optical module of the measurement head, characterized in that said beam is guided through an aperture located on an outer wall of the optical module.

44. A method according to claim 43, characterized in that said beam is focused substantially in said aperture.

45. A method according to claim 43, characterized in that it comprises a measurement of two emission beams substantially simultaneously.

46. A method according to claim 45, characterized in that the emission beam received from the sample is divided into two emission beams in the optical module and said two emission beams are guided through separate apertures of the optical module.

47. A method according to claim 43, characterized in that a part of the light from the illumination source is reflected, guided through an aperture of the optical module, and measured, and illuminating effect of the illumination source is determined on the basis of said measuring.

48. A method according to claim 47, characterized in that the part of the light from the illumination source is reflected with a dichroic mirror within the optical module, wherein the transmission/reflection properties of said third dichroic mirror are application specific.

49. A method according to claim 43, characterized in that the excitation beam is directed to the sample from above the sample, and the emission is measured from above the sample.

50. A method according to claim 43, characterized in that the excitation beam is directed to the sample from below the sample, and the emission is measured from below the sample.

51. A method according to claim 43, characterized in that the method comprises a photoluminescence measurement.