Embryonic stem cells, clinical applications and methods for expanding in vitro

• US 20030049233A1
• Filed: 10/25/2002
• Published: 03/13/2003
• Est. Priority Date: 07/18/2001
• Status: Abandoned Application

• Current Assignee: Medra, Inc.
• Original Assignee: Medra, Inc.
• Inventors: Rader, William C., Scheller, Albert
• Application Number:
  US10/280,745
• Publication Number:
  US 20030049233A1
• Time in Patent Office: Days
• US Class Current: 424/93.7
• CPC Class Codes: A61K 2035/124 the cells being hematopoietic, bone marrow derived or blood cells
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: C12N 2501/115 Basic fibroblast growth factor (bFGF, FGF-2)
• CPC Class Codes: C12N 2501/235 Leukemia inhibitory factor [LIF]
• CPC Class Codes: C12N 2503/00 Use of cells in diagnostics
• CPC Class Codes: C12N 5/0606 Pluripotent embryonic cells, e.g. embryonic stem cells [ES] embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696

Abstract

The present invention provides a method for the in vitro culture of embryonic stem cells, wherein the stem cells continue to express no antigen or antigen CD117, and mostly remain undifferentiated during culture. The present invention also relates to purified preparations of embryonic stem cells and for uses of embryonic stem cells in treating a wide variety of conditions, diseases and disorders.

Claims

1. A collection device for collecting cells suspended in a liquid medium, comprising:

a rod, wherein the rod is composed of a material that conducts electricity; and

a handle, wherein the handle is composed of a material that does not conduct electricity and wherein the handle is attached to one end of the rod.

2. A method for collecting and removing cells from a liquid medium, comprising the steps of:

providing a collection device comprising a rod and a handle, wherein the rod is composed of a material that conducts electricity, the handle is composed of a material that does not conduct electricity and the handle is attached to one end of the rod;

inserting the rod of the collection device into a liquid medium containing cells;

applying a charge to the surface of the rod, to thereby attract oppositely charged cells to attach onto the surface of the rod;

removing the charged rod from the liquid medium, to thereby remove the attached cells from the liquid medium;

placing the charged rod with the attached cells into a suitable receptacle for the cells; and

reversing the charge on the surface of the rod to thereby repel and detach the cells from the rod surface into the cell receptacle.

3. The method of claim 2 further comprising the step of rinsing the rod with a suitable liquid after placing the rod in the cell receptacle and reversing the charge on the rod surface, to thereby facilitate removal of the cells into the cell receptacle.