×

Enzymatic synthesis of oligonucleotide tags

  • US 20030049616A1
  • Filed: 01/08/2001
  • Published: 03/13/2003
  • Est. Priority Date: 01/08/2001
  • Status: Abandoned Application
First Claim
Patent Images

1. A method of synthesizing a repertoire of oligonucleotide tags of a predetermined length, the method comprising the steps of:

  • (a) providing a repertoire of oligonucleotide tag precursors in an amplicon, the oligonucleotide tag precursors each comprising one or more words, and each of the one or more words being selected from the same minimally cross-hybridizing set;

    (b) cleaving the amplicon at a word in each of the oligonucleotide tag precursors to form one or more ligatable ends on each oligonucleotide tag precursor;

    (c) ligating one or more words to the one or more ligatable ends to elongate each of the oligonucleotide tag precursors;

    (d) amplifying the elongated oligonucleotide tag precursors in the amplicon; and

    (e) repeating steps (b) through (d) until a repertoire of oligonucleotide tags having the predetermined length is formed.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×