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Electronically mediated nucleic acid amplification in NASBA

  • US 20030049632A1
  • Filed: 10/09/2001
  • Published: 03/13/2003
  • Est. Priority Date: 04/12/1999
  • Status: Active Grant
First Claim
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1. A method of amplifying specific target nucleic acids in a mixed sample of nucleic acids comprising:

  • a. introducing said target nucleic acids onto a bioelectronic microchip having a plurality of electronically addressable capture sites;

    b. electronically addressing said target nucleic acids to specific capture sites, said capture sites further having connected thereto capture probes that are specific for said target nucleic acids;

    c. hybridizing said target nucleic acids and said amplicons to said capture probes; and

    d. subjecting said target nucleic acids to a nucleic acid sequence-based amplification reaction to form amplicons of said target.

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