Use of non-invasive imaging technologies to monitor in vivo gene-expression
First Claim
1. A method of monitoring the effect of a compound on target gene expression in an animal, the method comprising:
- (a) providing an animal expressing a reporter gene operably linked to expression control elements associated with a target gene;
(b) administering a test compound to the animal; and
(c) non-invasively detecting a shift in resonance in the presence of the reporter gene product as being indicative of an increase or decrease in the target gene expression in the presence of the test compound.
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides methods of using non-invasive imaging technologies to monitor the effects of potential therapeutic compounds on gene expression in non-human living animals. This invention also provides methods to analyze perturbation in biochemical pathways and physiological functions associated with disease (e.g. a cancer, cardiovascular disease, degenerative nervous system disease, osteoporosis, disease related to body weight regulation, toxicity, and any other disease condition related to abnormal gene expression) using the non-invasive imaging technologies to monitor alterations in gene expression in non-human living animals.
32 Citations
30 Claims
-
1. A method of monitoring the effect of a compound on target gene expression in an animal, the method comprising:
-
(a) providing an animal expressing a reporter gene operably linked to expression control elements associated with a target gene;
(b) administering a test compound to the animal; and
(c) non-invasively detecting a shift in resonance in the presence of the reporter gene product as being indicative of an increase or decrease in the target gene expression in the presence of the test compound. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 27)
-
-
2. A method of monitoring the effect of a compound on target gene expression in an animal, the method comprising:
-
(a) providing an animal expressing a reporter gene operably linked to expression control elements associated with the target gene;
(b) administering a test compound to the animal;
(c) administering a recorder substrate to the animal;
(d) non-invasively detecting a shift in resonance of the recorder substrate as being indicative of an increase or decrease in the target gene expression in the presence of the test compound. - View Dependent Claims (26, 28)
-
-
29. A noninvasive method for detecting a level of gene expression in response to a compound, wherein said expression is regulated by alterations in cellular physiology, said method comprising:
-
(a) administering the compound to a transgenic animal under conditions that permit proton generation mediated by a proton generating compound associated with the gene product (b) placing the animal within a detection field of a proton detector device, (c) maintaining the animal in the detection field of the device, and (d) d measuring the proton emission in the animal with the proton detector device to detect the level of the proton generating compound the associated with a gene product whose expression regulated by alterations in cellular physiology, wherein an increase or decrease in the level of the proton generating compound is indicative of an alteration in gene expression in the presence of the compound. - View Dependent Claims (30)
-
Specification