Trans-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
First Claim
1. A method for effecting trans-differentiation of a somatic cell comprising (a) culturing a somatic cell in the presence of at least one agent selected from the group consisting of (a) cytoskeletal inhibitors and (b) inhibitors of acetylation, and (c) inhibitors of methylation;
- (b) culturing the cell in the presence of agents or conditions that induce differentiation to a cell type different than that of said somatic cell; and
(c) allowing the cell to trans-differentiate to said different cell type.
3 Assignments
0 Petitions
Accused Products
Abstract
The invention provides a method for effecting the trans-differentiation of a somatic cell, i.e., the conversion of a somatic cell of one cell type into a somatic cell of a different cell type. The method is practiced by culturing a somatic cell in the presence of at least one agent selected from the group consisting of (a) cytoskeletal inhibitors and (b) inhibitors of acetylation, and (c) inhibitors of methylation, and also culturing the cell in the presence of agents or conditions that induce differentiation to a different cell type. The method is useful for producing histocompatible cells for cell therapy.
-
Citations
20 Claims
-
1. A method for effecting trans-differentiation of a somatic cell comprising
(a) culturing a somatic cell in the presence of at least one agent selected from the group consisting of (a) cytoskeletal inhibitors and (b) inhibitors of acetylation, and (c) inhibitors of methylation; -
(b) culturing the cell in the presence of agents or conditions that induce differentiation to a cell type different than that of said somatic cell; and
(c) allowing the cell to trans-differentiate to said different cell type. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
-
-
16. A method for converting a non-neural somatic cell to become a cell having the phenotype of a cell of neural lineage, comprising:
-
(a) culturing a non-neural somatic cell in the presence of at least one agent selected from the group consisting of (a) cytoskeletal inhibitors and (b) inhibitors of acetylation, and (c) inhibitors of methylation;
(b) culturing the cell in the presence of agents or conditions that induce differentiation to a cell of neuronal lineage; and
(c) allowing the cell to trans-differentiate to a cell having the phenotype of a cell of neural lineage. - View Dependent Claims (17, 18, 19, 20)
-
Specification