Transduced marrow stromal cells
First Claim
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1. A method for transducing marrow stromal cells, said method comprising infecting marrow stromal cells with a vector which comprises a bicistronic coding region comprising a nucleic acid encoding tyrosine hydroxylase type I (TH) and a sequence encoding GTP cyclohydrolase I (GC), operably linked to a promoter/regulatory region, thereby transducing the marrow stromal cell.
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Abstract
The present invention embodies a method of transducing marrow stromal cells with retroviral vectors comprising the TH and GC enzyme precursors of L-DOPA. The invention also describes a method of producing exogenous L-DOPA using this transduction method. Novel retroviral vectors comprising TH and GC, with an intervening IRES are also described.
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Citations
18 Claims
- 1. A method for transducing marrow stromal cells, said method comprising infecting marrow stromal cells with a vector which comprises a bicistronic coding region comprising a nucleic acid encoding tyrosine hydroxylase type I (TH) and a sequence encoding GTP cyclohydrolase I (GC), operably linked to a promoter/regulatory region, thereby transducing the marrow stromal cell.
- 11. A vector construct comprising a nucleic acid encoding TH and GC separated by an internal ribosomal entry site (IRES).
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