Cell activation process and reagents therefor
First Claim
1. A method of activating a cell as a result of one type of extracellular interaction between said first cell and a molecule associated with a second target cell characterised in that said first cell is provided with a DNA delivery system comprising DNA coding for one or more recombinant chimeric receptors comprising two or more different cytoplasmic signalling components, wherein said cytoplasmic components are not naturally linked, and at least one is derived from a membrane spanning polypeptide, characterised in that at least one of said cytoplasmic signalling components is a co-stimulatory signalling domain derived from all or part of a tetra-span-transmembrane protein, CD43, CD6, a mannose, IL-7, IL-12 or complement receptor, an integrin-associated protein, or a γ
- -chain associated with a cytokine receptor.
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Accused Products
Abstract
A cell activation process is described in which an effector cell is transformed with DNA coding for a chimeric receptor containing two or more different cytoplasmic signalling components. At least one of the cytoplasmic signalling components is derived from all or part of a tetraspan-transmembrane protein, CD43, CD6, a mannose, U17, IL-12 or complement receptor, an integrin-associated protein, or a γ-chain associated with a cytokine receptor. The activated cell may be of use in medicine for example in the treatment of diseases such as cancer.
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Citations
52 Claims
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1. A method of activating a cell as a result of one type of extracellular interaction between said first cell and a molecule associated with a second target cell characterised in that said first cell is provided with a DNA delivery system comprising DNA coding for one or more recombinant chimeric receptors comprising two or more different cytoplasmic signalling components, wherein said cytoplasmic components are not naturally linked, and at least one is derived from a membrane spanning polypeptide, characterised in that at least one of said cytoplasmic signalling components is a co-stimulatory signalling domain derived from all or part of a tetra-span-transmembrane protein, CD43, CD6, a mannose, IL-7, IL-12 or complement receptor, an integrin-associated protein, or a γ
- -chain associated with a cytokine receptor.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 45, 46, 47)
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11. A DNA delivery system comprising DNA in association with a carrier said DNA coding for a recombinant chimeric receptor capable of one type of extracellular interaction and comprising two or more different cytoplasmic signalling components which are not naturally linked, and wherein at least one of said cytoplasmic components is derived from a membrane spanning polypeptide, characterised in that at least one of said cytoplasmic signalling components is a co-stimulatory signalling domain derived from all or part of a tetra-span-transmembrane protein, CD43, CD6, a mannose, IL-7, IL-12 or complement receptor, an integrin-associated protein, or a γ
- -chain associated with a cytokine receptor.
- View Dependent Claims (13, 14, 17, 19, 20, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 48, 49, 50, 51, 52)
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12. A DNA delivery system comprising DNA in association with a carrier said DNA coding for two or more recombinant chimeric receptors each capable of the same one type of extracellular interaction and wherein each of said receptors comprises one or more different cytoplasmic signalling components which are not naturally linked, and wherein at least one of said cytoplasmic components is derived from a membrane spanning polypeptide, characterised in that at least one of said cytoplasmic signalling components is a co-stimulatory signalling domain derived from all or part of a tetra-span-transmembrane protein, CD43, CD6, or a mannose, IL-7, IL-12 or complement receptor, an integrin-associated protein, or a γ
- -chain associated with a cytokine receptor.
- View Dependent Claims (15, 16, 18, 21, 22)
Specification