Probe array for detecting a target material using stereo-substrate

• US 20030064386A1
• Filed: 04/24/2002
• Published: 04/03/2003
• Est. Priority Date: 09/28/2001
• Status: Abandoned Application

• Current Assignee: Olympus Corporation
• Original Assignee: Olympus Optical Company Ltd. (Olympus Corporation)
• Inventors: Ohashi, Yoko, Karaki, Sachiko, Kano, Tokio
• Application Number:
  US10/128,955
• Publication Number:
  US 20030064386A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: G01N 33/54313 the carrier being characterised by its particulate form

Abstract

The present invention discloses a probe array for detection comprising at least one stereo-substrate and plural kinds of probes immobilized on the surface of the stereo-substrate, wherein the probes are respectively and specifically bonded with target materials which differ every kind.

Claims

1. A probe array for detection comprising a stereo-substrate and plural kinds of probes immobilized on the surface of the stereo-substrate, wherein the probes are respectively and specifically bonded with target materials which differ every kind.

2. A probe array for detection according to claim 1, wherein the stereo-substrate is a rotational body.

3. A probe array for detection according to claim 2, further comprising a marker which indicates a coordinate point on the surface of the stereo-substrate.

4. A probe array for detection according to claim 3, wherein the marker is selected from the group consisting of a convex portion and a concave portion.

5. A probe array for detection according to claim 4, wherein the target material is a nucleic acid and the probes are a nucleic acid having a sequence complementary to the target material.

11. A probe array for detection according to claim 1, wherein the stereo-substrates are a bar shape.

6. A probe array for detection in one unit mode, comprising a plurality of stereo-substrates and plural kinds of probes immobilized on the surface of the stereo-substrates, wherein the probes are respectively and specifically bonded with target materials which differ every kind.

7. A probe array for detection according to claim 6, further comprising first markers which indicate a coordinate point on the respective surfaces of the stereo-substrates.

8. A probe array for detection according to claim 7, wherein the first markers are selected from the group consisting of a convex portion and a concave portion.

9. A probe array for detection according to claim 8, wherein second markers to discriminate respectively the plurality of stereo-substrates are provided.

10. A probe array for detection according to claim 9, wherein the second markers are applied to the stereo-substrates based on the difference of optical physical property selected from the group consisting of optical transparency, reflectivity, refractivity and the degree of polarizability of the stereo-substrates.

12. A probe array for detecting a target material in an assay object material, comprising a stereo-substrate, probes immobilized on the surface of the stereo-substrate and having specific affinity with respect to the target material, wherein 2 or more probes among from a first probe which is specifically bonded with a first target material to an n-th probe which is specifically bonded with an n-th target material are immobilized on the stereo-substrate (wherein n is an integer of 2 or more).

13. A probe array for detection according to claim 12, wherein the stereo-substrate is a rotational body.

14. A probe array for detection according to claim 12, further comprising a marker which indicates a coordinate point on the surface of the stereo-substrate.

15. A probe array for detection according to claim 14, wherein the marker is selected from the group consisting of a convex portion and a concave portion.

16. A probe array for detection according to claim 14, wherein the target material is a nucleic acid and the probes are a nucleic acid having a sequence complementary to the target material.

17. A probe array for detection according to claim 12, wherein the stereo-substrate is a bar shape.

18. A probe array for detection according to claim 17, further comprising means selected from the group consisting of a heating body and a heat transmission body in the inside of the bar-shaped stereo-substrate.

19. A reaction container comprising a probe array for detection according to any one of claims 17 and 18 and a tube-shaped housing which accommodates the probe array for detection.

20. A probe array for detection according to claim 19, wherein the stereo-substrate is a nucleic acid and the probe is a nucleic acid having a sequence complementary to the target material.