Synthesis of spatially addressed molecular arrays
First Claim
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1. A method for forming a spatially addressable array of polymers immobilized on a solid support, comprising the steps of:
- (i) contacting an array of single molecules with one or more detectably labelled monomers, under conditions that permit incorporation of a monomer onto a molecule of the array, wherein the labelled monomer comprises a removable blocking group that prevents further monomer incorporation occurring;
(ii) removing non-incorporated monomers and detecing the label on the incorporated monomer;
(iii) removing the blocking group and any separate label; and
(iv) optionally repeating steps (i)-(iii) to form a single polymer of defined sequence;
wherein the array has a surface density which allows each polymer to be individually resolved by optical microscopy.
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Abstract
Methods are disclosed for forming spatially addressable arrays of polynucleotides of known squence, by using blocking groups that prevent the incorporation of multiple nucleotides during each incorporation step.
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Citations
10 Claims
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1. A method for forming a spatially addressable array of polymers immobilized on a solid support, comprising the steps of:
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(i) contacting an array of single molecules with one or more detectably labelled monomers, under conditions that permit incorporation of a monomer onto a molecule of the array, wherein the labelled monomer comprises a removable blocking group that prevents further monomer incorporation occurring;
(ii) removing non-incorporated monomers and detecing the label on the incorporated monomer;
(iii) removing the blocking group and any separate label; and
(iv) optionally repeating steps (i)-(iii) to form a single polymer of defined sequence;
wherein the array has a surface density which allows each polymer to be individually resolved by optical microscopy. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification