Method for the synthesis of DNA sequences
First Claim
1. A method for the fabrication of a DNA construct of target sequence comprising the steps of defining a plurality of overlapping single stranded DNA sequence segments which in the aggregate would make up the target DNA sequence;
- making a DNA microarray on a substrate in which single stranded DNA molecules constructed in the microarray correspond to the overlapping sequence segments;
releasing the overlapping single stranded DNA segments from the substrate; and
allowing the overlapping single stranded DNA segments to assemble into the target DNA construct.
1 Assignment
0 Petitions
Accused Products
Abstract
A method is disclosed for the direct synthesis of double stranded DNA molecules of a variety of sizes and with any desired sequence. The DNA molecule to be synthesis is logically broken up into smaller overlapping DNA segments. A maskless microarray synthesizer is used to make a DNA microarray on a substrate in which each element or feature of the array is populated by DNA of a one of the overlapping DNA segments. The DNA segments are released from the substrate and held under conditions favoring hybridization of DNA, under which conditions the segments will spontaneously hybridize together to form the desired DNA construct. This method makes possible the remote assembly of DNA sequence, through a process analogous to facsimile transmission of documents, since the information on DNA to be made can be transmitted remotely to an instrument which can then synthesize any needed DNA sequence from the information.
114 Citations
14 Claims
-
1. A method for the fabrication of a DNA construct of target sequence comprising the steps of
defining a plurality of overlapping single stranded DNA sequence segments which in the aggregate would make up the target DNA sequence; -
making a DNA microarray on a substrate in which single stranded DNA molecules constructed in the microarray correspond to the overlapping sequence segments;
releasing the overlapping single stranded DNA segments from the substrate; and
allowing the overlapping single stranded DNA segments to assemble into the target DNA construct. - View Dependent Claims (2, 3, 4, 5)
-
-
6. A method for the fabrication of a target DNA construct of comprising the steps of
defining a plurality of overlapping single stranded DNA sequence segments which in the aggregate make up the target DNA construct; -
making a the overlapping single stranded DNA molecules in an array on a substrate;
releasing the overlapping single stranded DNA segments from the substrate; and
allowing the overlapping single stranded DNA segments to assemble into the target DNA construct. - View Dependent Claims (7, 8, 9, 10, 11, 12)
-
-
13. A method for the fabrication of a DNA construct of target sequence comprising the steps of
defining a plurality of overlapping single stranded DNA sequence segments which in the aggregate would make up the target DNA sequence; -
making a DNA microarray on a substrate in which single stranded DNA molecules constructed in the microarray correspond to the overlapping sequence segments;
releasing the overlapping single stranded DNA segments from the substrate;
allowing the overlapping single stranded DNA segments to assemble into the target DNA construct; and
ligating the assembled DNA segments into a DNA molecule.
-
-
14. A composition of the formula:
Specification