Methods and compositions for the construction and use of fusion libraries

• US 20030068649A1
• Filed: 03/12/2002
• Published: 04/10/2003
• Est. Priority Date: 09/14/2000
• Status: Abandoned Application

• Current Assignee: Xencor, Inc.
• Original Assignee: Xencor, Inc.
• Inventors: Doberstein, Stephen K., Li, Min, Melander, Christian, Jin, Cheng He, Liu, Hong-Xiang
• Application Number:
  US10/097,100
• Publication Number:
  US 20030068649A1
• Time in Patent Office: Days
• US Class Current: 435/7.1
• CPC Class Codes: C12N 15/1034 Isolating an individual clone by screening libraries
• CPC Class Codes: C12N 15/1062 mRNA-Display, e.g. polypeptide and encoding template are connected covalently
• CPC Class Codes: C12N 15/1075 by coupling phenotype to genotype, not provided for in other groups of this subclass
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching

Abstract

This invention pertains to genetic libraries encoding enzyme fusion proteins and methods of use to identify a nucleic acid of interest.

Claims

1. A library of nucleic acid/protein (NAP) conjugates each comprising:

a) a fusion polypeptide comprising;

i) a NAM enzyme; and

ii) a candidate protein;

b) an expression vector comprising

i) a fusion nucleic acid comprising;

1) a nucleic acid encoding said NAM enzyme; and

2) a nucleic acid encoding said candidate protein;

 

wherein at least two of said candidate proteins are different; and

,

c) an enzyme attachment sequence (EAS), wherein said EAS is an RNA sequence;

 

wherein said EAS and said NAM enzyme are covalently attached.

3. A library according to claim 1 or 2 wherein said NAM enzyme is a Rep protein.

4. A library according to claim 1 or 2 wherein said Rep protein is a Rep 68 protein.

5. A library according to claim 1 or 2 wherein said Rep protein is a Rep 78 protein.

2. A library of expression vectors each comprising:

a) a fusion nucleic acid comprising;

i) a nucleic acid encoding a NAM enzyme; and

,

ii) a nucleic acid encoding a candidate protein;

 

wherein at least two of said candidate proteins are different; and

,

b) a DNA binding motif that is recognized by a small molecule conjugate.

6. A method of making a library of fusion polypeptides comprising:

a) providing a first fusion nucleic acid comprising;

i) a nucleic acid encoding a NAM enzyme; and

ii) a nucleic acid encoding a ligation mediating moiety;

b) providing a second fusion nucleic acid comprising;

i) a nucleic acid encoding a candidate protein; and

ii) a nucleic acid encoding a ligation substrate;

 

wherein at least two of said candidate proteins are different;

c) ligating said first and said second fusion nucleic acids to form fusion nucleic acids comprising a Rep protein and a candidate protein; and

,

d) expressing said fusion nucleic acids under conditions whereby a library of fusion polypeptides are formed wherein said fusion polypeptides comprise a NAM enzyme and a candidate protein.

7. A method according to claim 6 wherein said ligation substrate is ubiquitin.

8. A method of making a library of fusion polypeptides comprising:

a) providing a first fusion nucleic acid comprising;

i) a nucleic acid encoding a NAM enzyme; and

ii) a nucleic acid encoding an N-terminal intein motif;

b) providing a second fusion nucleic acid comprising;

i) a nucleic acid encoding a candidate protein; and

ii) a nucleic acid encoding a C-terminal intein motif;

 

wherein at least two of said candidate proteins are different.

c) combining said first and said second fusion nucleic acids under conditions whereby protein splicing occurs; and

,

d) forming a library of fusion polypeptides comprising a NAM enzyme and a candidate protein.

9. A method of making a library of fusion polypeptides comprising:

a) providing;

i) an acceptor donor substrate comprising a NAM enzyme wherein said NAM enzyme comprises at least one reactive glutamine residue;

ii) a donor candidate protein comprising at least one lysine residue;

b) combining said NAM enzyme and said candidate protein under conditions whereby transglutaminase is active; and

,

c) forming a NAM enzyme-candidate protein fusion.

10. A library of expression vectors comprising:

a) a fusion nucleic acid comprising;

i) a nucleic acid encoding a NAM enzyme; and

ii) a nucleic acid encoding a candidate protein;

b) an enzyme attachment sequence (EAS) that is recognized by said NAM enzyme; and

c) a recombination system.

11. A method of detecting the presence of a target analyte in a sample comprising:

a) providing a biochip comprising an array of candidate target analytes;

b) contacting said array with a library of nucleic acid/protein (NAP) conjugates comprising;

i) a fusion polypeptide comprising;

1) a NAM enzyme; and

2) a candidate protein;

ii) an expression vector comprising;

1) a fusion nucleic acid comprising;

A) nucleic acid encoding said NAM enzyme;

B) nucleic acid encoding said candidate protein; and

C) an enzyme attachment sequence (EAS);

 

wherein said EAS and said NAM enzyme are covalently attached, under conditions wherein at least one of said candidate target analytes can bind to at least one of said candidate proteins to form an assay complex; and

c) detecting the presence of said assay complex on said substrate.

12. A method for screening a library of small molecules comprising:

a) providing a biochip comprising an array of small molecule targets;

b) contacting said array with a library of NAP conjugates comprising;

i) a fusion polypeptide comprising;

1) a NAM enzyme; and

2) a candidate protein;

ii) an expression vector comprising;

1) a fusion nucleic acid comprising;

A) nucleic acid encoding said NAM enzyme;

B) nucleic acid encoding said candidate protein; and

C) an enzyme attachment sequence (EAS);

 

wherein said EAS and said NAM enzyme are covalently attached, under conditions wherein at least one of said small molecule targets can bind to at least one of said candidate proteins to form an assay complex;

c) screening said array under conditions wherein at least one of said small molecule targets can bind to at least one of said NAP conjugates to form an assay complex; and

d) detecting the presence of said assay complex on said substrate.

13. A method according to claim 12 further comprising deconvoluting and identifying said NAP conjugates.