Low volume filtration column devices and methods of filtering therewith

• US 20030069413A1
• Filed: 07/30/2002
• Published: 04/10/2003
• Est. Priority Date: 06/15/2001
• Status: Active Grant

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Richardson, Derrick, Monteon, Jorge, Kunitake, Steven T., Pai, Derek S.
• Granted Patent: US 7,749,388 B2
• Time in Patent Office: Days
• US Class Current: 536/25.4
• CPC Class Codes: B01L 2300/042 Caps; Plugs
• CPC Class Codes: B01L 2300/043 Hinged closures
• CPC Class Codes: B01L 2300/046 Function or devices integrated in the closure
• CPC Class Codes: B01L 2300/048 enabling gas exchange, e.g. vents
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 3/5021 Test tubes specially adapted for centrifugation purposes centrifuges B04B5/04
• CPC Class Codes: B01L 3/5082 Test tubes per se
• CPC Class Codes: G01N 1/40 Concentrating samples
• CPC Class Codes: G01N 1/405 by adsorption or absorption

Abstract

This relates to filter columns for isolating nucleic acids, particularly at small elution volumes. The filter column is adapted for stable placement within the upper portion of standard plastic collection tubes of various sizes. The body of the filter column has a number of surfaces to accommodate placement within variously sized collection tubes. The filter column contains nucleic acid-specific filter which can be located at alternate regions within the filter column, providing different filter surface areas and loading volume capacities using the same column body. The filter column has an opening on an upper end adapted to be sealed by a cap. A method for recovering nucleic acids using such filter column is also provided.

Claims

1. A filter column, comprising:

a body having a passageway extending therethrough, the body having a first end, a second end and a longitudinal axis, an outer surface and an inner surface;

the outer surface having at least one bearing surface for seating on at least one collection tube;

the inner surface having at least one angled surface located proximate or above the at least one bearing surface;

the at least one angled surface forming an angle with the longitudinal axis that is less than approximately 90 degrees; and

a filter disposed within the passageway.

2. The filter column of claim 1 wherein the outer surface further includes a second bearing surface for seating on at least a second collection tube that has a different size from the at least one collection tube.

3. The filter column of claim 1 further including at least a first body portion and a second body portion;

wherein at least one bearing surface is located between the first body portion and the second body portion; and

4. The filter column of claim 3 wherein at least one angled surface is located between the first body portion and the second body portion.

5. The filter column of claim 3 wherein the second body portion is configured to be removably locatable within a collection tube having a volume of approximately 1.5 to 2.0 milliliters.

6. The filter column of claim 3 wherein the cross-sectional area of the passageway at the first body portion is greater than the cross-sectional area of the passageway at second body portion.

7. The filter column of claim 3 further including a third body portion;

wherein at least one bearing surface is located between the second body portion and the third body portion.

8. The filter column of claim 7 wherein at least one angled surface is located between the second body portion and the third body portion.

9. The filter column of claim 7 wherein the third body portion is configured to be removably locatable within a collection tube having a volume of approximately 0.5 milliliters.

10. The filter column of claim 7 wherein the passageway at the third body portion has a cross-sectional area;

the cross-sectional area of the passageway at the second body portion being greater than the cross-sectional area of the passageway at the third body portion.

11. The filter column of claim 3 wherein the filter is located in the second body portion.

12. The filter column of claim 7 wherein the filter is located in the third body portion.

13. The filter column of claim 1 further including a filter support configured to support the filter.

14. The filter column of claim 13 wherein the filter support is a porous membrane.

15. The filter column of claim 14 wherein the filter support has a substantially smooth upper surface.

16. The filter column of claim 1 further including a lid adapted to mate with the first end of the body.

17. The filter column of claim 16 wherein at least one vent is defined in the body at the first end.

18. The filter column of claim 1 wherein the inner surface is configured such that fluid disposed into the filter column does not pool on the angled surface while stationary or while undergoing centrifugation.

19. The filter column claim 1 wherein the at least one angled surface forms an angle with the longitudinal axis that is approximately 45 degrees or less.

20. The filter column of claim 1 wherein each of the bearing surfaces is adapted to seat on respective collection tubes of different sizes.

21. The filter column of claim 1 wherein the first end of the body is adapted to mate with a sample carrier.

22. A filter column, comprising:

a body having a passageway extending therethrough, the body having an outer surface, an inner surface and a longitudinal axis;

a filter disposed within the passageway;

the filter defining a perimeter; and

a retainer disposed within the passageway above the filter;

the retainer having an outer edge, an inner edge, and an upper surface that is beveled.

23. The filter column of claim 22 wherein the upper surface is beveled such that the upper surface forms an angle with the longitudinal axis of less than approximately 90 degrees.

24. The filter column of claim 22 wherein the upper surface is beveled such that fluid disposed into the filter column does not pool on the upper surface while stationary or while undergoing centrifugation.

25. The filter column of claim 22 wherein the retainer has a lower surface that is beveled.

26. The filter column of claim 25 wherein the upper and lower surfaces of the retainer are beveled such that the thickness of the retainer decreases with distance from the outer edge towards the inner edge.

27. The filter column of claim 22 wherein at least a portion of the filter is compressed by the retainer.

28. The filter column of claim 27 wherein the at least a portion of the filter that is compressed by the retainer is the outer edge of the filter.

29. The filter column of claim 22 wherein the retainer is adapted to compress the filter such that at least a portion of the filter proximate to the perimeter is compressed.

30. The filter column of claim 22 further including a filter support.

31. The filter column of claim 22 wherein the outer surface has at least two bearing surfaces for seating on collection vessels of at least two different sizes.

32. The filter column of claim 22 wherein the retainer is ring-shaped.

33. A filter column, comprising:

a body having a passageway extending therethrough, the body having an outer surface, an inner surface, and a longitudinal axis; and

a filter disposed within the passageway, the filter having a perimeter;

wherein at least a portion of the filter proximate to the perimeter is compressed relative to a portion of the filter that is inward from the perimeter.

34. The filter column of claim 33 further including a retainer disposed within the passageway above the filter.

35. The filter column of claim 34 wherein the at least a portion of the filter proximate to the perimeter is compressed by the insertion of the retainer.

36. The filter column of claim 33 wherein the filter has an original filter height and wherein the at least a portion of the filter is compressed by at least approximately 10 percent of the original filter height.

37. The filter column of claim 33 wherein the filter has an original filter height and wherein the at least a portion of the filter is compressed by at least approximately 30 percent of the original filter height.

38. The filter column of claim 33 wherein the filter has an original filter height and wherein the at least a portion of the filter is compressed by at least approximately 50 percent of the original filter height.

39. The filter column of claim 33 further including a filter support disposed beneath the filter.

40. The filter column of claim 33 further including at least two bearing surfaces such that the filter column is adapted to seat on collection tubes of at least two different sizes.

41. A filter column, comprising:

a body having a passageway extending therethrough, the body having, an outer surface, an inner surface, and a longitudinal axis; and

a filter disposed within the passageway, the filter having a perimeter;

wherein at least a portion of the filter has a greater density relative to another portion of the filter.

42. The filter column of claim 41 wherein the at least a portion of the filter of greater density is proximate to the perimeter relative to a portion of the filter that is inward from the perimeter.

43. The filter column of claim 41 wherein the filter includes a compression profile such that the compression of the filter gradually increases with distance towards the outer edge.

44. The filter column of claim 43 further including a retainer that is disposed above the filter;

the greater density portion of the filter resulting from the retainer compressing the filter.

45. The filter column of claim 41 wherein the filter includes an original density;

the at least a portion of the filter of greater density is at least approximately 30 percent more dense than the original density of the filter.

46. A filter column for isolating nucleic acids into solution of a preselected elution volume, comprising:

a body having a passageway extending therethrough, the body having a first end, a second end, an outer surface and an inner surface; and

at least one filter disposed within the passageway;

the filter having a wetting capacity;

wherein the wetting capacity of the filter is approximately equal to the elution volume.

47. The filter column of claim 46 further including at least a first body portion and a second body portion;

the cross-sectional area of the passageway at the first body portion being greater than the cross-sectional area of the passageway at the second body portion.

48. The filter column of claim 46 further including at least two bearing surfaces such that the filter column is adapted to seat on collection tubes of at least two different sizes.

49. The filter column of claim 46 wherein the wetting capacity is approximately equal to the preselected elution volume such that the wetting capacity is ±

10 percent of the elution volume.

50. The filter column of claim 46 wherein the wetting capacity is approximately 8 to 12 microliters.

51. A filter column for isolating nucleic acids into solution of a preselected elution volume, comprising:

a body having a passageway extending therethrough, the body having a first end, a second end, an outer surface and an inner surface; and

at least one filter disposed within the passageway;

the filter having a wetting capacity;

wherein the wetting capacity of the filter is less than the preselected elution volume.

52. The filter column of claim 51 wherein the wetting capacity is approximately 50 percent to 100 percent of the preselected elution volume.

53. The filter column of claim 51 wherein the wetting capacity is approximately 66 percent to 100 percent of the preselected elution volume.

54. A method for isolating nucleic acid material, comprising:

providing a filter column;

the filter column comprising;

a body having a passageway extending therethrough, the body having an outer surface and an inner surface;

at least one filter disposed within the passageway, the filter having a wetting capacity;

providing a solution containing nucleic acid material;

transferring the solution containing nucleic acid to the filter of the filter column;

transferring solution from the filter column;

adding elution buffer to the filter in an amount substantially equal to the wetting capacity of the filter; and

eluting the nucleic acid from the filter column.

55. The method of claim 54 wherein the step of transferring the solution containing nucleic acid to the filter of the filter column further includes transfer the solution containing nucleic acid to the filter of the filter column in the presence of an agent capable of promoting binding of the nucleic acid material to the filter.

56. The method of claim 54 further including the step of washing the filter column with a wash solution.

57. The method of claim 54 further including the step of adding additional elution buffer to the filter column in an amount that is between zero and approximately fifty percent of the wetting capacity of the filter.

58. The method of claim 54 further including the step of adding additional elution buffer to the filter column in an amount that is between zero and approximately 66 percent.

59. A filter column for isolating nucleic acids into solution of a preselected elution volume, comprising:

a body having a passageway extending therethrough, the body having a first end, a second end, an outer surface, and an inner surface;

a filter disposed within the passageway;

the filter having an shape that defines a volume;

wherein the filter is configured such that the volume defined by the shape of the filter is substantially equal to the preselected elution volume.

60. A method for isolating a nucleic acid material, comprising:

providing a filter column;

the filter column comprising;

a body having a passageway extending therethrough, the body having an outer surface and an inner surface;

a filter disposed within the passageway, the filter having a shape that defines a volume;

providing a solution containing nucleic acid material;

transferring the solution containing nucleic acid to the filter;

transferring the solution from the filter column;

adding elution buffer to the filter column in an amount substantially equal to the volume defined by the shape of the filter; and

eluting the nucleic acid from the filter column.

61. The method of claim 60 wherein the step of transferring the solution containing nucleic acid to the filter further includes transferring the solution containing nucleic acid to the filter in the presence of an agent capable of promoting binding of the nucleic acid material to the filter.

62. The method of claim 60 further including the step of washing the filter column with a wash solution.

63. A method for isolating a nucleic acid material comprising:

providing a filter column;

the filter column comprising;

a body having a passageway extending therethrough, the body having an outer surface and an inner surface;

a filter disposed within the passageway;

the filter having a wetting capacity;

providing solution containing nucleic acid material in the filter column;

locating the filter column in fluid communication with a first collection vessel;

transferring the solution containing nucleic acid to the filter of the filter column;

transferring the solution from the filter column at a first speed followed by a second speed wherein the first speed is slower than the second speed;

adding elution buffer into the filter column; and

eluting the nucleic acid material from the filter column.

64. The method of claim 63 wherein the step of transferring the solution containing nucleic acid to the filter further includes transferring the solution containing nucleic acid to the filter in the presence of an agent capable of promoting binding of the nucleic acid material to the filter.

65. The method of claim 63 wherein the first speed is approximately 500 g or less.

66. The method of claim 63 wherein the first speed is approximately 1000 g or less.

67. The method of claim 63 wherein the first speed is approximately 1500 g or less.

68. The method of claim 63 wherein the first speed is approximately 2000 g or less.

69. The method as in one of claims 63-68 wherein the second speed is approximately 10,000 g or more.

70. The method of claim 63 the step of transferring the solution from the filter column is performed via centrifugal force.

71. The method of claim 63 wherein the step of providing a filter column further includes providing a filter column having at least two bearing surfaces formed in the outer surface such that the filter column is adapted to seat on collection vessels of at least two different sizes;

the method further including the step of seating the filter column on the first collection vessel; and

wherein the step of transferring the solution containing nucleic acid from the filter column includes transferring the solution containing nucleic acid to a first collection vessel.

72. The method of claim 63 wherein the step of adding elution buffer includes adding elution buffer in an amount that is substantially equal to the wetting capacity of the filter.

73. The method of claim 71 further including the steps of:

removing the filter column from the first collection vessel;

seating the filter column on a second collection vessel; and

wherein the step of eluting the nucleic acid material from the filter column includes eluting the nucleic acid material into the second collection vessel.

74. The method of claim 73 wherein the second collection vessel is smaller than the first collection vessel.

75. The method of claim 63 further including the step of washing the filter with a wash solution.

76. The method of claim 63 wherein the step of eluting the nucleic acid material from the filter column is performed a first speed followed by a second speed wherein the first speed is slower than the second speed.

77. The method of claim 76 wherein the first speed of the elution step is approximately 500 g or less.

78. The method of claim 76 wherein the first speed of the elution step is approximately 1000 g or less.

79. The method of claim 76 wherein the first speed of the elution step is approximately 1500 g or less.

80. The method of claim 76 wherein the first speed of the elution step is approximately 2000 g or less.

81. The method as in one of claims 76-80 wherein the second speed of the elution step is approximately 10,000 g or more.

82. The method of claim 63, wherein the solution containing a nucleic acid material consists of a lysate solution of prepared from biological material disrupted with a disruption solution.

83. A method for isolating a nucleic acid material comprising:

providing a filter column;

the filter column comprising;

a body having a passageway extending therethrough, the body having an outer surface and an inner surface;

a filter disposed within the passageway;

providing solution containing nucleic acid material in the filter column;

locating the filter column in fluid communication with a first collection vessel;

transferring the solution containing nucleic acid to the filter of the filter column;

transferring solution from the filter column;

adding elution buffer into the filter column; and

eluting the nucleic acid material from the filter column at a first speed followed by a second speed wherein the first speed is slower than the second speed.

84. The method of claim 83 wherein the step of transferring the solution containing nucleic acid to the filter of the filter column further includes transferring the solution containing nucleic acid to the filter of the filter column in the presence of an agent capable of promoting binding of the nucleic acid material to the filter.

85. The method of claim 83 wherein the first speed of the elution step is approximately 500 g or less.

86. The method of claim 83 wherein the first speed of the elution step is approximately 1000 g or less.

87. The method of claim 83 wherein the first speed of the elution step is approximately 1500 g or less.

88. The method of claim 83 wherein the first speed of the elution step is approximately 2000 g or less.

89. The method as in one of claims 83-88 wherein the second speed of the elution step is approximately 10,000 g or more.

90. The method of claim 83 wherein the step of transferring the solution from the filter column is performed via centrifugal force.

91. The method of claim 83 wherein the step of providing a filter column further includes providing a filter column having at least two bearing surfaces formed in the outer surface such that the filter column is adapted to seat on collection vessels of at least two different sizes;

the method further including the step of seating the filter column on a first collection vessel; and

wherein the step of transferring the solution containing nucleic acid from the filter column includes transferring the solution containing nucleic acid to a first collection vessel.

92. The method of claim 91 further including the steps of:

removing the filter column from the first collection vessel;

locating the filter column in fluid communication with a second collection vessel; and

wherein the step of eluting the nucleic acid material from the filter column includes eluting the nucleic acid material into the second collection vessel.

93. The method of claim 92 wherein the second collection vessel is smaller than the first collection tube.

94. The method of claim 83 further including the step of washing the filter with a wash solution.

95. The method of claim 83 wherein the step of transferring solution from the filter is performed at a first speed followed by a second speed wherein the first speed is slower than the second speed.

96. The method of claim 95 wherein the first speed of the transfer step is approximately 500 g or less.

97. The method of claim 95 wherein the first speed of the transfer step is approximately 1000 g or less.

98. The method of claim 95 wherein the first speed of the transfer step is approximately 1500 g or less.

99. The method of claim 95 wherein the first speed of the transfer step is approximately 2000 g or less.

100. The method as in one of claims 95-99 wherein the second speed of the transfer step is approximately 10,000 g or more.

101. The method of claim 83, wherein the solution containing a nucleic acid material consists of a lysate solution of prepared from biological material disrupted with a disruption solution.

102. A filter column, comprising:

a body having a passageway extending therethrough;

the body having a first end, a second end, an outer surface, and an inner surface;

the outer surface including at least two bearing surfaces for seating the filter column on collection vessels of at least two different sizes; and

a filter disposed within the passageway;

wherein the cross-sectional area of the passageway is substantially constant.