Peelable and resealable devices for arraying materials

• US 20030073228A1
• Filed: 07/29/2002
• Published: 04/17/2003
• Est. Priority Date: 11/08/2000
• Status: Active Grant

• Current Assignee: Surface Logix, Inc.
• Original Assignee: Surface Logix, Inc.
• Inventors: Kirk, Gregory L., Campbell, Stewart, Duffy, David, Schueller, Olivier, Agosto, Melina
• Granted Patent: US 7,439,056 B2
• Time in Patent Office: Days
• US Class Current: 435/287.1
• CPC Class Codes: B01D 2325/08 Patterned membranes
• CPC Class Codes: B01J 2219/00274 Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00662 Two-dimensional arrays within two-dimensional arrays
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/00743 Cells
• CPC Class Codes: B01L 2200/021 Adjust spacings in an array of wells, pipettes or holders, format transfer between arrays of different size or geometry
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0848 Specific forms of parts of containers
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/123 Flexible; Elastomeric
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 5/00 : Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C12M 23/12 : Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12Q 1/025 : for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics antimicrobial activity C12Q1/18
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 15/1468 : with spatial resolution of the texture or inner structure of the particle
• CPC Class Codes: G01N 2015/1497 : Particle shape
• CPC Class Codes: G01N 33/54366 : Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G06T 2207/10008 : from scanner, fax or copier
• CPC Class Codes: G06T 2207/10064 : Fluorescence image
• CPC Class Codes: G06T 2207/30072 : Microarray; Biochip, DNA array; Well plate
• CPC Class Codes: G06T 7/0012 : Biomedical image inspection
• CPC Class Codes: G06T 7/70 : Determining position or orientation of objects or cameras camera calibration G06T7/80

Abstract

Devices and methods for performing assays on materials, particularly biological materials, are provided. The devices and methods make use of self-sealing members, which can be applied to a flat surface to form wells to facilitate immobilization of materials on the flat surface, then removed to yield a flat surface that facilitates the performance of processes on and/or detection of the immobilized material.

Claims

1. A device for arraying a biological material comprising:

a base plate;

a removable member defining at least one orifice therein and having walls bounding the at least one orifice on sides of the at least one orifice, the removable member being in self-sealing contact with the base plate for defining at least one well therewith corresponding to a respective one of the

at least one orifice, the removable member being further adapted to repeatedly self-seal on the base plate;

at least one biological material immobilized within a corresponding one of the at least one well.

2. The device of claim 1, wherein the at least one orifice comprises a plurality of orifices defining an array of orifices, and the at least one well comprises a plurality of wells, and the at least one biological material being immobilized on the base plate within each of the plurality of wells in an array corresponding to the array of orifices.

3. The device of claim 2, wherein the at least one biological material comprises a single biological material.

4. The device of claim 2, wherein the at least one biological material comprises at least two biological materials disposed in respective ones of at least two of the plurality of wells.

5. The device of claim 2, wherein the at least one biological materials comprises a plurality of biological materials immobilized within at least one of the plurality of wells.

6. The device of claim 2, wherein the plurality of orifices defines an array that spatially and dimensionally corresponds to a standard array of wells in an industry standard microtiter plate.

7. The device of claim 6, wherein the standard microtiter plate is selected from the group consisting of a 6-well microtiter plate, a 12-well microtiter plate, a 24-well microtiter plate, a 96-well microtiter plate, a 384-well microtiter plate, a 1,536-well microtiter plate, and a 9,600-well microtiter plate.

8. The device of claim 1, wherein the base plate is made of a material selected from the group consisting of glass, silicon, fused silica, metal films, polystyrene, poly(methylacrylate), and polycarbonate.

9. The device of claim 1, wherein the base plate has a substantially flat upper surface, the at least one biological material being immobilized on the flat upper surface of the base plate.

10. The device of claim 1, further comprises a first layer of metal disposed on an upper surface of the base plate.

11. The device of claim 10, wherein the first layer of metal made of a material is selected from the group consisting of gold, silver, platinum, palladium and copper.

12. The device of claim 11, further comprising a second layer of metal disposed between the upper surface of the base plate and the first layer of metal.

13. The device of claim 12, wherein the second layer of metal is made of a material selected from the group consisting of chromium and titanium.

14. The device of claim 10, wherein the first layer has a substantially flat upper surface, the at least one biological material being immobilized on the flat upper surface of the first layer.

15. The device of claim 14, wherein the upper surface of the first layer is biologically inert.

16. The device of claim 9, wherein the upper surface of the base plate is treated for having a self-assembling monolayer disposed thereon.

17. The device of claim 1, further comprising further comprising at least one registration element adapted to align with one another the base plate and the removable member.

18. The device of claim 17, wherein the at least one registration element comprises a registration pin adapted to extend through the base plate, the first removable member, and the second removable member for aligning respective ones of the base plate, the first removable member and the second removable member.

19. The device of claim 17, wherein the at least one registration element includes a visual mark disposed on at least one of the base plate and the removable member.

20. The device of claim 1, wherein the removable member is made of PDMS.

21. The device of claim 1, wherein the walls of the removable member bounding the at least one orifice are tapered in a direction from an upper surface of the removable member toward a lower surface of the removable member such that an opening of the at least one orifice is larger in the upper surface of the removable member than an opening of the at least one orifice in the lower surface of the removable member.

22. The device of claim 1, wherein the removable member is a first removable member, the at least one orifice is at least one first orifice, and the at least one well is at least one first well, the device further comprising a second removable member defining at least one second orifice therein and having walls bounding the at least one second orifice on sides of the at least one second orifice, the second removable member being in self-sealing contact with at least one of the base plate and the first removable member for defining at least one second well therewith corresponding to a respective one of the at least one second orifice, the second removable member further being adapted to repeatedly self-seal on the at least one of the base plate and the first removable member.

23. The device of claim 22, wherein the at least one second orifice comprises a plurality of second orifices defining an array of second orifices, and the at least one second well comprises a plurality of second wells.

24. The device of claim 23, wherein the array of second orifices spatially and dimensionally corresponds to a standard array of wells in an industry standard microtiter plate.

25. The device of claim 24, wherein the standard microtiter plate is selected from the group consisting of a 6-well microtiter plate, a 12-well microtiter plate, a 24-well microtiter plate, a 96-well microtiter plate, a 384-well microtiter plate, a 1,536-well microtiter plate, and a 9,600-well microtiter plate.

26. The device of claim 22, wherein:

the at least one first orifice comprises a plurality of first orifices defining an array of first orifices; and

the array of second orifices spatially and dimensionally corresponds to the array of first orifices.

27. The device of claim 23, wherein:

the at least one first orifice comprises a plurality of first orifices defining an array of first orifices; and

at least one of the plurality of second orifices is larger than at least one of the plurality of first orifices.

28. The device of claim 23, wherein the at least one second well encompasses at least one first well.

29. The device of claim 23, wherein:

the at least one first well comprises a plurality of first wells; and

the at least one second well encompasses a plurality of the plurality of first wells.

30. The device of claim 23, wherein the walls of the second removable member bounding the at least one second orifice are tapered in a direction from an upper surface of the second removable member toward a lower surface of the second removable member such that an opening of the at least one second orifice is larger in the upper surface of the second removable member than an opening of the at least one second orifice in the lower surface of the second removable member.

31. The device of claim 21, wherein the walls of the second removable member bounding the at least one second orifice are tapered in a direction from an upper surface of the second removable member toward a lower surface of the second removable member such that an opening of the at least one second orifice is larger in the upper surface of the second removable member than an opening of the at least one second orifice in the lower surface of the second removable member, a degree of tapering of the walls of the first removable member being substantially equal to a degree of tapering of the walls of the second removable member.

32. The device of claim 31, wherein the first removable member and the second removable member being configured such that the opening of the at least one first orifice registers with the opening of the at least one second orifice such that the walls bounding said the at least one first orifice form a continuous surface with the walls bounding said the at least one second orifice.

33. The device of claim 31, wherein the at least one second orifice exposes at least a portion of an upper surface of the first removable member.

34. The device of claim 31, wherein the at least one second orifice exposes at least a portion of an upper surface of the first removable member.

35. The device of claim 22, further comprising at least one registration element adapted to align with one another respective ones of the base plate, the first removable member and the second removable member.

36. The device of claim 35, wherein the at least one registration element comprises a registration pin adapted to extend through the base plate, the first removable member, and the second removable member for aligning respective ones of the base plate, the first removable member and the second removable member.

37. The device of claim 35, wherein the at least one registration element includes a visual mark disposed on at least one of the base plate and the removable member.

38. The device of claim 1 wherein the base plate is treated to enhance immobilization of peptide substrates thereon.

39. The device of claim 1 wherein the base plate is treated to enhance immobilization of kinase substrates thereon.

40. The device of claim 1, wherein the base plate is treated to enhance immobilization of antibody substrates thereon.

41. The device of claim 39, wherein the base plate is treated with a solution of mixed self-assembled monolayer (SAM), such that binding of kinase substrates is enhanced in discrete areas and reduced or eliminated in others.

42. The device of claim 41, wherein the base plate is treated with a solution of mixed SAM, such that specific binding of kinase substrates in desired orientations is enhanced and non-specific binding of kinase substrates is reduced or eliminated.

43. The device of claim 42, wherein the base plate is treated with mixed SAM comprising about 0.1 to 20% maleimide-terminal groups in a background of tri(ethylene glycol) terminal groups.