Clonal human embryonic stem cell lines and methods of generating same
First Claim
1. A method of establishing a clonal embryonic stem cell line capable of sustaining a phenotype of normal embryonic stem cells following at least eight months of in vitro culturing, the method comprising culturing an individual embryonic stem cell for at least eight months in a serum-free medium, thereby establishing the clonal embryonic stem cell line capable of sustaining said phenotype of normal embryonic stem cells following at least eight months of in vitro culture.
1 Assignment
0 Petitions
Accused Products
Abstract
A method of establishing a clonal embryonic stem cell line capable of sustaining a phenotype of normal embryonic stem cells following at least eight months of in vitro culture is disclosed. The method is effected by culturing an individual embryonic stem cell for at least eight months in a serum-free medium, thereby establishing the clonal embryonic stem cell line capable of sustaining said phenotype of normal embryonic stem cells following at least eight months of in vitro culture.
-
Citations
24 Claims
- 1. A method of establishing a clonal embryonic stem cell line capable of sustaining a phenotype of normal embryonic stem cells following at least eight months of in vitro culturing, the method comprising culturing an individual embryonic stem cell for at least eight months in a serum-free medium, thereby establishing the clonal embryonic stem cell line capable of sustaining said phenotype of normal embryonic stem cells following at least eight months of in vitro culture.
-
6. The method of claim 37 wherein said non-decreasing telomere length is selected from a range of 8 to 12 kb.
- 17. A clonal human embryonic stem cell line being capable of sustaining a normal embryonic stem cell phenotype following at least eight months of in vitro culturing.
-
24. A clonal human embryonic stem cell line being capable of sustaining a normal embryonic stem cell phenotype following at least twelve months of in vitro culturing.
Specification