Method and apparatus for solid state molecular analysis
First Claim
1. A method for the construction of a molecular deposition domain on a surface, comprising:
- (a) providing a surface;
(b) depositing a deposition material on a deposition device;
(c) depositing the deposition material on the surface using said deposition device, forming a molecular deposition domain smaller than one micron in total area.
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Abstract
The invention is a method for the formation and analysis of novel miniature deposition domains. These deposition domains are placed on a surface to form a molecular array. The molecular array is scanned with an AFM to analyze molecular recognition events and the effect of introduced agents on defined molecular interactions. This approach can be carried out in a high throughput format, allowing rapid screening of thousands of molecular species in a solid state array. The procedures described here have the added benefit of allowing the measurement of changes in molecular binding events resulting from changes in the analysis environment or introduction of additional effector molecules to the assay system. The processes described herein are extremely useful in the search for compounds such as new drugs for treatment of undesirable physiological conditions. The method and apparatus of the present invention does not require the labeling of the deposition material or the target sample and may also be used to deposit large size molecules without harming the same.
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Citations
66 Claims
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1. A method for the construction of a molecular deposition domain on a surface, comprising:
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(a) providing a surface;
(b) depositing a deposition material on a deposition device;
(c) depositing the deposition material on the surface using said deposition device, forming a molecular deposition domain smaller than one micron in total area. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
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38. A method for constructing an array of molecular deposition domains comprising
(a) providing a surface; -
(b) providing an at least one deposition material;
(c) depositing a first deposition material on a deposition device;
(d) depositing the first deposition material on the surface in a known position, forming a first molecular deposition domain smaller than one micron in total area;
(e) cleaning the deposition device;
(f) repeating the above steps to form an at least one other deposition domain, creating an array of two or more deposition domains on said surface. - View Dependent Claims (39)
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40. A method for detecting a target sample, the method comprising:
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(a) forming a molecular array on a surface, the molecular array including an at least one molecular deposition domain, said at least one molecular deposition domain smaller than one micron in total area;
(b) exposing the surface to a sample medium, the sample medium containing one or more target samples which cause a molecular interaction event in one or more of the at least one deposition domain;
(c) scanning the surface using a scanning probe microscope to detect the occurrence of the molecular interaction event caused by the target sample. - View Dependent Claims (41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55)
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56. A molecular array for characterizing molecular interaction events, comprising:
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(a) a surface; and
(b) an at least one molecular deposition domain deposited on said surface wherein the spatial address of the domain is less than one micron in area. - View Dependent Claims (57, 58, 59, 60, 61, 62, 63, 64, 65)
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66. A method for the processing of an array comprising:
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(a) forming an array on a substrate, the array comprising a plurality of deposition domains formed of a deposition material;
(b) exposing the array to one or more materials which contain an at least one target sample that causes a molecular interaction event with one or more of the deposition samples; and
(c) scanning the array utilizing a scanning probe microscope to characterize the molecular interaction events that have occurred between the target sample and the deposition material.
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Specification