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Methods and compositions for enhancing sensitivity in the analysis of biological-based assays

  • US 20030077595A1
  • Filed: 10/24/2001
  • Published: 04/24/2003
  • Est. Priority Date: 01/23/1996
  • Status: Active Grant
First Claim
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1. A method for detecting the binding of a first member to a second member of a ligand pair, comprising:

  • (a) combining a set of first tagged members with a biological sample which may contain one or more second members, under conditions, and for a time sufficient to permit binding of a first member to a second member, wherein said tag is correlative with a particular first member and detectable by non-fluorescent spectrometry or potentiometry;

    (b) separating bound first and second members from unbound members;

    (c) cleaving said tag from said tagged first member; and

    (d) detecting said tag by non-fluorescent spectrometry or potentiometry, and therefrom detecting the binding of said first member to said second member.

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