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Methods and systems for dynamic gene expression profiling

  • US 20030077611A1
  • Filed: 04/01/2002
  • Published: 04/24/2003
  • Est. Priority Date: 10/24/2001
  • Status: Abandoned Application
First Claim
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1. A method for comparing gene expression profiles of two or more samples, said method comprising:

  • (a) synthesizing a plurality of first strand cDNAs from a first sample using a first oligonucleotide primer comprising a sample-specific sequence tag, wherein said sample-specific sequence tag is GC rich at its 5′

    terminal and At rich at its 3′

    terminal;

    (b) selectively amplifying at least a subset of said cDNA so as to generate one or more sample-specific amplified products;

    (c) detecting the abundance of one or more said sample-specific amplified products, wherein said abundance determines an expression profile of one or more genes in said first sample; and

    (d) comparing the expression profile of said one or more genes in said first sample with an expression profile of said one or more genes in a second sample, wherein a difference in the expression profile indicates differential expression of said one or more genes in the two samples.

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