Method for formulating a glucose oxidase enzyme with a desired property or properties and a glucose oxidase enzyme with the desired property
First Claim
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1. A method for formulating an enzyme comprising:
- obtaining at least one glucose oxidase gene;
creating at least one mutated glucose oxidase gene;
introducing each mutated glucose oxidase gene into separate expression vectors;
inserting the expression vectors into host organisms;
growing colonies of the host organisms; and
screening the colonies for desirable properties.
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Abstract
A method for formulating a glucose oxidase enzyme with peroxide resistant properties and a glucose oxidase enzyme formulated by the method. The enzyme formulation method results in a glucose oxidase enzyme with improve resistance to peroxide, and therefore, with improved resistance to oxidative inactivation. The method employs directed evolution techniques to evolve glucose oxidase to achieve the desirable properties. A peroxide resistant glucose oxidase may improve the longevity of, for example, glucose biosensors in which a peroxide resistant glucose oxidase may be placed.
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Citations
43 Claims
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1. A method for formulating an enzyme comprising:
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obtaining at least one glucose oxidase gene;
creating at least one mutated glucose oxidase gene;
introducing each mutated glucose oxidase gene into separate expression vectors;
inserting the expression vectors into host organisms;
growing colonies of the host organisms; and
screening the colonies for desirable properties. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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26. A method for formulating an enzyme comprising:
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obtaining an organism with a glucose oxidase gene;
growing multiple colonies of the organism;
altering the environment of the colonies; and
screening the colonies to identify colonies with active glucose oxidase after altering the environment of the colonies. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43)
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Specification