Differentiation of whole bone marrow
First Claim
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1. A method to generate a clinically significant volume of neural progenitor cells, the method comprising:
- providing a mass of cells obtained from a mammal including at least one stem cell; and
culturing the at least one stem cell in a medium comprising fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF) to produce a clinically significant volume of neural progenitor cells.
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Abstract
A method is described for generating a clinically significant volume of neural progenitor cells from whole bone marrow. A mass of bone marrow cells may be grown in a culture supplemented with fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF). Further methods of the present invention are directed to utilizing the neural progenitor cells cultured in this fashion in the treatment of various neuropathological conditions, and in targeting delivery of cells transfected with a particular gene to diseased or damaged tissue.
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Citations
52 Claims
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1. A method to generate a clinically significant volume of neural progenitor cells, the method comprising:
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providing a mass of cells obtained from a mammal including at least one stem cell; and
culturing the at least one stem cell in a medium comprising fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF) to produce a clinically significant volume of neural progenitor cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method to treat a neuropathologic condition, the method comprising:
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providing a mass of cells obtained from a mammal including at least one stem cell;
culturing the at least one stem cell in a medium comprising fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF) to produce a neural progenitor cell; and
administering the neural progenitor cell to a mammal. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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34. A method to deliver a biological compound, the method comprising:
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providing a mass of cells obtained from a mammal including at least one stem cell;
culturing the at least one stem cell in a medium comprising fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF) to produce a neural progenitor cell;
causing the neural progenitor cell to carry a gene that effects local production of the biological compound; and
administering the neural progenitor cell to a mammal. - View Dependent Claims (35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52)
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Specification