Biological assays using coded RNA reporters
First Claim
1. A method for multiplexed analysis of reporter gene activity, comprising:
- introducing a first reporter gene into a first cell population;
introducing a second reporter gene into a second cell population, wherein the first and second reporter genes express distinguishable first and second RNA reporters, respectively; and
measuring the levels of the first and second RNA reporters associated with the first and second cell populations, respectively.
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Accused Products
Abstract
Systems for multiplexed analysis of gene expression using coded RNA reporters. The coded RNA reporters are expressed from a library of reporter genes, with each reporter gene directing expression of a distinguishable coded RNA reporter. Each RNA reporter thus reports activity of a corresponding reporter gene. The distinguishable reporter RNA species allow otherwise similar reporter genes to be functionally linked to different effectors within a cell reporter library. Multiplexed analysis of reporter gene activity is carried out by measuring the level of each RNA reporter within the cell reporter library, thus providing an ability to analyze the effect of a biological modulator on many effectors.
8 Citations
22 Claims
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1. A method for multiplexed analysis of reporter gene activity, comprising:
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introducing a first reporter gene into a first cell population;
introducing a second reporter gene into a second cell population, wherein the first and second reporter genes express distinguishable first and second RNA reporters, respectively; and
measuring the levels of the first and second RNA reporters associated with the first and second cell populations, respectively. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification